Publication: Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis
Authors
Ruiz-González, Irene ; Sánchez, M.A. ; García-Fernández, R.A. ; García-Palencia, P. ; Sánchez, B. ; González-Bulnes, A. ; Flores, J.M.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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Description
Abstract
The present study compares two protocols
for ovine estrus synchronization by assessing the
caruncular angiogenic response to the establishment of
pregnancy. The analysis consisted of the immunohistochemical
evaluation of Vascular Endothelial
Growth Factor (VEGF), Platelet Endothelial Cell
Adhesion Molecule-1 (PECAM-1, CD31) and Von
Willebrand Factor (vWF) in ovine caruncular stroma. A
flock of thirty-eight adult ewes was divided in two
groups and synchronized with either progestagens
(Group P) or prostaglandin analogues (Group PG).
Immunohistochemistry was performed in uterine
samples obtained from pregnant ewes (P, n=15; PG,
n=13) on days 15 post coitus (pc), 17pc and 21pc (day 0
=day of estrus). Each factor was assessed by total
vascular density (TVD, total positive blood
vessels/mm2), capillary vascular density (CVD, positive
blood capillaries/mm2) and arteriolar vascular density
(AVD, positive arterioles/mm2). Group P demonstrated
higher VEGF-CVD (P=0.045) when compared to
prostaglandin treated animals. Vascular CD31-
expression decreased on days 15pc and 21pc (TVD,
P=0.007 and CVD, P=0.014) in both groups. vWF
analysis did not show significant differences between
groups or days of study. These results demonstrate a
different influence of progestagen-based and
prostaglandin analogues-based synchronization
treatments over VEGF vascular expression during
caruncular development taking place in response to
pregnancy establishment. In addition, observations
pointed out in this study support the involvement of
CD31 in the angiogenic stimulus that occurs during early
maternal placentation in the ewe.
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Citation
Histology and histopathology, Vol. 28, n.º 3 (2013)
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