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Effects of pumpkin and fermented whey on fecal microbiota profile against AFB1 and OTA exposure in Wistar rats

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dc.contributor.authorLázaro, Álvaro
dc.contributor.authorVila-Donat, Pilar
dc.contributor.authorCimbalo, Alessandra
dc.contributor.authorManyes, Lara
dc.contributor.authorGómez Ramírez, Pilar
dc.contributor.departmentCiencias Sociosanitariases
dc.date.accessioned2025-07-09T10:55:00Z
dc.date.available2025-07-09T10:55:00Z
dc.date.copyright© 2025 informa UK limited, trading as Taylor & Francis Group.
dc.date.issued2025-04-08
dc.description© 2025 informa UK limited, trading as Taylor & Francis group. "Once the embargo has passed this document Will be available under the CC-BY-NC 4.0 license. http://creativecommons.org/licenses/by-nc/4.0/”. This document is the Accepted version of a Published Work that appeared in final form in Toxicology Mechanisms and Methods. To access the final edited and published work see https://doi.org/10.1080/15376516.2025.2484636
dc.description.abstractMycotoxins perturb the gut microbiota performance. Bioactive compounds have been recently used as a new food strategy to diminish mycotoxins bioaccessibility and prevent their toxic effects on human and animal health. Male and female Wistar rats were exposed orally to twelve different diets containing aflatoxin B1 (aFB1) and/or ochratoxin a (Ota) with or without fermented whey (FW) and pumpkin (P) for 28 days. Fecal microbiota using 16s rRNa gene sequencing and subsequent metagenomics analysis were analyzed to study the effect of 28-day exposure through diet of contaminated and enriched feed. QiiMe 2 microbiome analysis package (version 2024.5) was used to analyze the demultiplexed data. Mycotoxins-functional ingredients combination contributed more to microbial phylogenetic faith α-diversity rather than the functional ingredients alone, while the same combination reported a microbial α-diversity enhancement in comparison to the mycotoxins alone. Proteobacteria phylum was reduced in rat samples fed with contaminated diets (aFB1, Ota, and aFB1+Ota), while there was an increase— although not in all groups—when adding the functional ingredients. the main difference between the sexes was found in FW+aFB1+Ota group, with males (25%) showing higher % of proteobacteria than females (1.86%). Phylogenetic diversity faith only focuses on microbial genetic (dis)similarity, not considering the biological function. Morganella morganii, a roteobacteria found in some groups presents anticancer activity, but it is also related to inflammatory bowel disease and colorectal cancer. to sum up, both mycotoxins and functional ingredients trigger changes in the microbiota profile of Wistar rats in a sex-specific manner.
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dc.format.extent14
dc.identifier.citationToxicology Mechanisms and Methods, 2025
dc.identifier.doihttps://doi.org/10.1080/15376516.2025.2484636
dc.identifier.issnPrint: 1537-6516
dc.identifier.issnElectronic: 1537-6524
dc.identifier.urihttp://hdl.handle.net/10201/157141
dc.languageenges
dc.publisherTaylor and Francis Group, Taylor and Francis
dc.relationThis research was funded by spanish Ministry of science, innovation and Universities for the project PiD2022-140722OB-i00, MciU/ aei/10.13039/501100011033/FeDeR, eU and for the postdoctoral grant of Alessandra Cimbalo ‘Margarita salas’ (Ms21-165).es
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.1080/15376516.2025.2484636
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectMycotoxins
dc.subjectProbiotics
dc.subjectFeces
dc.subjectMicrobiota
dc.subjectSexual dimorphism
dc.titleEffects of pumpkin and fermented whey on fecal microbiota profile against AFB1 and OTA exposure in Wistar ratses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/acceptedVersión
dspace.entity.typePublicationes
relation.isAuthorOfPublicationcfe66521-035d-4f55-b5c0-5ee24d9b11d3
relation.isAuthorOfPublication.latestForDiscoverycfe66521-035d-4f55-b5c0-5ee24d9b11d3
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