Publication:
Circ_0031242 regulates the functional properties of hepatocellular carcinoma cells through the miR-944/MAD2L1 axis

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Lin-38-303-316-2023.pdf(14.66 MB)
Date
2023
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Authors
Lin, Jianwei ; Lin, Zenghai ; Hua, Yaqiong ; Chen, Yan
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-519
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info:eu-repo/semantics/article
Description
Abstract
Background. Circular RNAs (circRNAs) possess key functions in the pathogenesis of hepatocellular carcinoma (HCC). Nonetheless, the actions of individual circRNAs in HCC remain undefined. Methods. circ_0031242, miR-944, and MAD2L1 expression were quantified by qRT-PCR. Transwell assay was utilized to examine cell invasion and migration. Glucose consumption and lactate production were measured to assess the impact on glycolysis. The relationships among circ_0031242, MAD2L1, and miR944 were examined via luciferase reporter assay. Results. circ_0031242 was notably augmented in HCC. Loss of function of circ_0031242 hindered cell proliferation, invasion, migration, glycolysis, and promoted apoptosis, as well as impeding HCC tumor growth. circ_0031242 directly targeted miR-944. Inhibition of miR-944 counteracted the effects of sicirc_0031242 on HCC cells. Additionally, miR-944 was proved to directly target MAD2L1 in HCC cells. Moreover, the promotion of MAD2L1 was able to rescue the inhibition of high miR-944 expression on HCC cell progression. Meanwhile, circ_0031242 involved the post-transcriptional modulation of MAD2L1 through miR-944. Conclusion. This study suggested that circ_0031242 regulated tumor cell progression and tumor growth through the miR-944/MAD2L1 axis in HCC.
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circ_0031242 , miR-944 , MAD2L1 , HCC , Cell progression , Glycolysis
Citation
Histology and Histopathology Vol. 38, nº3 (2023)
URI
http://hdl.handle.net/10201/129503
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Histology and histopathology Vol.38, nº3 (2023)
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