Publication: Dendritic cells and interleukin-2,cytochemical and ultrastructural study
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Date
2000
Authors
Civallero, M. ; Barni, Sergio ; Nano, R. ; Capelli, E.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim of the present study was to verify
the effect of IL-2 on dendritic cell (DC) differentiation.
Various cytokines have been indicated as factors
inducing DC differentiation, but no data about the
interleukin-2 (IL-2) effect on DC differentiation have
been reported. Monocytes isolated from peripheral blood
were treated in vitro with the following factors: IL-2, IL-
4, GM-CSF and G-CSF alone or in combination.
Morphological (also ultrastructural) and cytochemical
observations were carried out starting from 3 to 21 days
of treatment. The results indicate that the differentiation
of cells showing dendritic pattern is related to the
presence of IL-2. Moreover a synergic effect of IL-2 and
GM-CSF was observed. The enzymatic features changed
with the culture time: before the differentiation into DC,
the stimulated cells expressed the typical pattern of
monocytes. On the contrary, at advanced stage of
differentiation, some enzyme activities changed and in
terminally differentiated dendritic cells the reactions for
peroxidase and serine esterase were negative.
Considering the morphological features, the ability
to interact with lymphocytes and the enzymatic pattern
observed, we suppose that IL-2 may act as a maturative
factor rather than as a growth factor in the DC
differentiation.
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