Publication: Distribution and expression of mRNAs for the proto-oncogenes c-fos and c-jun in bone cells in vivo
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Date
1998
Authors
Oyama, M. ; Chiba, J. ; Kato, Y. ; Igarashi, N. ; Yoshida, M. ; Ishigami, M. ; Sugawara, S. ; Kobayashi, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In this study we assessed the expression and
localization of the proto-oncogenes c-fos and c-jun in
normal bone so as to gain more insight into the role of
these proto-oncogenes in bone tissue. Femurs of 4-weekold
rats were examined by non-radioactive in situ
hybridization. cDNA probes for c-fos- and c-jun-labeled
digoxygenin were produced by Polymerase Chain
Reaction (PCR).
C-fos and c-jun exhibited similar distribution in
growth plate and bone tissue. Expression of c-fos and cjun
mRNAs in growth plate was observed in the
proliferative zone and partly in the upper layer of the
hypertrophic zone. In spongy bone, high expression of cfos
and c-jun mRNAs was observed in the osteoblast
cytoplasm. However, there was little expression in bone
lining cells. In the bony trabeculae, slight expression of
c-fos and c-jun was observed in the premature osteocytes
situated close to the bone surface, but no expression was
detected in osteocytes that possessed relatively large
lacunae in the center of the trabeculae. C-fos and c-jun
were also slightly expressed in osteoclasts. These data
strongly suggest that c-fos and c-jun are involved in
regulating chondrocyte proliferation as immediate early
genes, and may also be involved in the gene expression
of bone matrix proteins as transcription factor (M-1) in
vivo. In addition, the fact that strong expression was
observed in osteoblasts but hardly any expression at all
in bone lining cells seems to suggest that these genes are
also involved in osteoblast activation.
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