Electrochemical biosensor for glycine detection in biological fluids

Wang, Qianyu; Yujie, Liu; Campillo-Brocal, Jonatan C.; Jiménez-Quero, Amparo; Crespo, Gaston A.; Cuartero, María

Publication:
Electrochemical biosensor for glycine detection in biological fluids

dc.contributor.author	Wang, Qianyu
dc.contributor.author	Yujie, Liu
dc.contributor.author	Campillo-Brocal, Jonatan C.
dc.contributor.author	Jiménez-Quero, Amparo
dc.contributor.author	Crespo, Gaston A.
dc.contributor.author	Cuartero, María
dc.contributor.department	Genética y Microbiología
dc.date.accessioned	2024-02-08T11:14:40Z
dc.date.available	2024-02-08T11:14:40Z
dc.date.issued	2021-03-11
dc.description	© 2021 The Author(s). Published by Elsevier B.V. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Biosensors and Bioelectronics. To access the final edited and published work see https://doi.org/ 10.1016/j.bios.2021.113154	es
dc.description.abstract	We present herein the very first amperometric biosensor for the quantitative determination of glycine in diverse biological fluids. The biosensor is based on a novel quinoprotein that catalyzes the oxidation of glycine with high specificity. This process is coupled to the redox conversion of Prussian blue in the presence of hydrogen peroxide originating from the enzymatic reaction. The optimized tailoring of the biosensor design consists of the effective encapsulation of the quinoprotein in a chitosan matrix with the posterior addition of an outer Nafion layer, which is here demonstrated to suppress matrix interference. This is particularly important in the case of ascorbic acid, which is known to influence the redox behavior of the Prussian blue. The analytical performance of the biosensor demonstrates fast response time (<7 s), acceptable reversibility, reproducibility, and stability (<6% variation) as well as a wide linear range of response (25–500 μM) that covers healthy (and even most unhealthy) physiological levels of glycine in blood/serum, urine and sweat. A total of 6 real samples from healthy patients and animals were analyzed: two serum, two urine and two sweat samples. The results were validated via commercially available fluorescence kit, displaying discrepancy of less than 9% in all the samples. The unique analytical features and effortless preparation of the new glycine biosensor position it at the forefront of current technologies towards decentralized clinical applications and sport performance monitoring.	es
dc.format	application/pdf	es
dc.format.extent	9	es
dc.identifier.citation	Biosensors and Bioelectronics 182 (2021) 113154
dc.identifier.doi	https://doi.org/10.1016/j.bios.2021.113154
dc.identifier.issn	Print: 0956-5663
dc.identifier.issn	Electronic: 1873-4235
dc.identifier.uri	http://hdl.handle.net/10201/138968
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska- Curie grant agreement No. 792824	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Glycine biosensor	es
dc.subject	Quinoprotein	es
dc.subject	Prussian blue	es
dc.subject	Biological fluids	es
dc.subject	Point-of-care-sensing	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología	es
dc.title	Electrochemical biosensor for glycine detection in biological fluids	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es