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Circular RNA circ_SKA3 enhances gastric cancer development by targeting miR-520h

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dc.contributor.authorWang, Chuntao
dc.contributor.authorJiang, Hao
dc.contributor.authorPeng, Jiaqun
dc.contributor.authorWeng, Duanshun
dc.contributor.authorZhang, Yu
dc.contributor.authorZhou, Yanxun
dc.contributor.authorZhang, Qin
dc.date.accessioned2023-03-21T08:32:49Z
dc.date.available2023-03-21T08:32:49Z
dc.date.issued2023
dc.description.abstractPurpose. To explore the mechanisms of action of circ_SKA3 in gastric cancer (GC), which are still not fully understood. Methods. Subcellular localization assay was used to analyze the localization of circ_SKA3, and Actinomycin D assay was applied to confirm the stability of circ_SKA3. The levels of circ_SKA3, microRNA (miR)- 520h, and cell division cycle 42 (CDC42) mRNA were gauged by quantitative real-time polymerase chain reaction (qRT-PCR). The protein levels of CDC42 and proliferating cell nuclear antigen (PCNA) were assessed by western blot. Cell proliferation, colony formation, cell cycle distribution, apoptosis, migration, and invasion were detected by 3-[4,5-dimethylthiazol-2-yl]- 2, 5-diphenyltetrazolium bromide (MTT), 5-Ethynyl-2’- Deoxyuridine (EdU) incorporation, colony formation, flow cytometry, and transwell assays, respectively. Directed relationship between miR-520h and circ_SKA3 or CDC42 was verified by a dual-luciferase reporter assay. Mouse xenograft experiments were used to elucidate the impact of circ_SKA3 in vivo. Results. Overexpression of circ_SKA3 was validated in GC tissues and cells. The down-regulation of circ_SKA3 suppressed proliferation, cell cycle progression, colony formation, migration, invasion, and promoted cell apoptosis in vitro, as well as weakening tumor growth in vivo. Circ_SKA3 directly bound to miR-520h, and circ_SKA3 regulated CDC42 expression through miR-520h. Circ_SKA3 exerted regulatory effects on GC cell behaviors by inhibiting miR-520h. Furthermore, CDC42 was a functional target of miR520h in regulating GC cell behaviors. Conclusion. Our findings established a strong molecular mechanism, the miR-520h/CDC42 axis, at least in part, for the oncogenic role of circ_SKA3 in GC.es
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dc.format.extent12es
dc.identifier.citationHistology and Histopathology Vol. 38, nº3 (2023)
dc.identifier.doihttps://doi.org/ 10.14670/HH-18-521
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/129504
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectGastric cancer (GC)es
dc.subjectcirc_SKA3es
dc.subjectmiR520hes
dc.subjectCdc42es
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleCircular RNA circ_SKA3 enhances gastric cancer development by targeting miR-520hes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.38, nº3 (2023)