Publication: Post-Thaw quality of spermatozoa frozen with three different extenders in the Murciano Granadina goat breed.
Authors
Galián, S. ; Peinado, B. ; Almela, L. ; Poto, A. ; Ruiz, S.
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Publisher
MDPI
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DOI
https://doi.org/10.3390/ani13020309
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info:eu-repo/semantics/article
Description
© 2023. The authors. This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/
This document is the accepted version of a published work that appeared in final form in
Animals
Abstract
In caprine species, the semen quality of thawed samples still needs to be improved
in order for this type of semen to provide an acceptable pregnancy rate when it is used for artificial
insemination. It is therefore essential to have an adequate freezing protocol and extender for this
species. By adapting freezing protocols that have proven successful in other species, such as pigs,
together with the development of an extender that protects the sperm cells from damage caused
by the freezing and thawing processes, and taking into account its composition in nutrients and
cell-membrane-stabilizing substances, it has been possible, at least in vitro, to achieve high sperm
quality in buck semen. In vivo studies of the fertilizing capacity of the semen cryopreserved in this
way will be the next step to verify the effectiveness of the extender and semen-freezing protocol
described in this work.
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Citation
Animals 2023, 13, 309
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