Publication: Expression of myostatin in early postnatal mouse masseter and rectus femoris muscles
Authors
Takada, Hiroshi ; Miwa, Yoko ; Sato, Iwao
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
10.14670/HH-11-631
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info:eu-repo/semantics/article
Description
Abstract
Aims: Myostatin (Mstn) is a member of the
transforming growth factor-β (TGF-β) family that
inhibits muscle differentiation. In this study, we aimed to
identify the relationships between Mstn, thyroid
hormone receptor alpha (TRα), and myosin heavy chain
(MyHC) isoform expression during early postnatal
development. Methods: We investigated the expression
of Mstn, TRα, and MyHCs (embryonic, slow, IIa, IIb,
and IIx) using quantitative real-time RT-PCR and ELISA
(Mstn) in postnatal mouse muscles between day 0 and
day 10. We also examined the correlations between
Mstn, TR and MyHCs during the early development of
mouse masseter muscle (MM) and rectus femoris muscle
(RFM). Results: Distinct Mstn mRNA expression
patterns were observed in the two muscles despite nearly
non-significant changes in the Mstn protein abundance
in MM. The expression pattern of the TRα mRNA in the
MM differed from that observed in the RFM. The
expression of MyHC IIa, IIb and IIx mRNAs increased
in the MM and decreased in the RFM from day 0 to day
10, whereas embryonic fiber MyHC mRNA expression
was similar in both muscle types. Principal component
analysis showed the existence of a correlation between:
(1) TRα and MyHC, (2) Mstn and MyHC, and (3) TRα
and Mstn in MM. The correlations were different in
RFM and MM.
Cluster analyses identified the distinct clusters:
cluster 1, days 0-4 for the MM and day 0 for the RFM;cluster 2, day 6 for the MM and day 2 for the RFM; and
cluster 3, days 8-10 for the MM and days 4-10 for the
RFM. Conclusions: These data suggest that TRα
influences MyHC expression in both muscle types. In
addition, Mstn has a limited effect in the MM related to
the expression of individual MyHCs, as opposed to its
role in the RFM, at early postnatal developmental stages.
TRα could be involved in regulating both the temporal
expression of MyHCs and Mstn at the early postnatal
stages in the MM and RFM.
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Citation
Histology and Histopathology, vol.30, nº 11, (2015)
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