Publication: Combined analysis of tumor growth pattern and expression of endogenous lectins as a prognostic
tool in primary testicular cancer and its lung metastases
Authors
Kayser, K. ; Hoeft, D. ; Hufnagl, P. ; Caselitz, J. ; Zick, Y. ; André, S. ; Kaltner, H. ; Gabius, H.J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim of this study was to glyco- and
immunohistochemically analyze expression of distinct
growth/adhesion-related markers of primary testicular
carcinomas and their lung metastases in relation to the
risk of developing lung metastases and survival of
patients, and to correlate immunohistochemical staining
profile and syntactic structure analysis in order to
delineate new prognostic parameters for this tumor type.
Clinical features of 50 patients with primary testicular
carcinomas and their corresponding lung metastases
were evaluated and compared to those of a control
cohort of 25 cases. The set of eight probes including
labeled galectins-1 and -3, specific non-cross-reactive
antibodies against galectins-1, -3, and -8 as well as anti-
Ki-67, anti-bcl-2, and anti-p53 was applied to formalinfixed,
paraffin-embedded tumor sections of both primary
and metastatic lesions. Syntactic structure analysis
computed staining intensities and structural features of
the tumor cells. These parameters were set into relation
separately and in combination to clinical data including
tumor stages, smoking habits, applied cytostatic therapy,
disease-free interval, and survival. The risk of testis
cancer patients to develop lung metastases depends in
descending order on the tumor cell type (non-seminoma
versus seminoma), tumor cell heterogeneity (mixed
versus monomorphous cell type), age of patients, and pT
stage. The extent of differential expression of galectinrelated
features between primary and secondary lesions was pronounced. Prognostic correlations for distinct
galectin-related features were delineated in combination
with data from syntactic structure analysis, for example
cluster radius of galectin-3-positive tumor cells and postsurgical
and total survival. Lengths of disease-free
interval and total survival of patients were also
correlated to characteristics obtained by syntactic structure analysis and their combination with galectin
data in the first place, then to smoking habits, percentage
of proliferating cells in the primary and secondary
tumors, and finally to expression of certain galectins and
of p53. Patients with non-seminoma testicular cancer
should be thoroughly controlled for lung metastases.
Regarding marker selection, our study underscores that
further investigation of the growth-regulatory network of
galectins is clearly warranted.
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