Publication: Transcription regulators are transiently expressed during the prostate gland adaptation to the hypoandrogenic environment
Authors
Nishan, Umar ; Rosa Ribeiro, Rafaela ; Lenz Cesar, Carlos ; Carvalho, Hernandes F.
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
publication.page.editor
publication.page.department
DOI
DOI: 10.14670/HH-18-105
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The high incidence of prostatic diseases,
including malignant tumors, makes the understanding of
prostate biology very important. Androgen deprivation,
blockade by orchiectomy, or chemical castration causes
prostate and tumor shrinkage. The gene networks
involved in a cell type-specific fashion are rather
unknown. This work was undertaken to identify genes
with annotated function in transcription regulation that
might define transitions in gene expression. A total of 15
potential regulatory genes were identified. Validation by
qRT-PCR showed that Zfp703 and Arid1a exhibit
expression maxima at day 1; Ash2l, Nelf, Pbx3, Eya2 at
day 4; Dmrt2 at day 5 and Lbh and Sox1 at day 7 after
castration. Using immunohistochemistry, we further
determined that PBX3 was found in both stromal and
epithelial cells, whereas ARID1A and NELF were
restricted to the epithelium, and DMRT2 and EYA2 were
exclusively found in the stroma. Though the proteins
ZFP703 and ASH2l were not found in any experimental
condition, their mRNAs were located by in situ
hybridization in both epithelium and stroma. In
conclusion, androgen deprivation triggers the expression
of temporally regulated gene sets in both epithelial and
stromal cells. These gene subsets will help establish the
regulatory gene expression programs orchestrating the
castration-induced remodeling of the prostate gland, and
represent putative targets to increase the efficacy of
androgen-deprivation to induce epithelial (and cancer)
cell death.
publication.page.subject
Citation
Histology and Histopathology, Vol.34, nÂş9, (2019)
item.page.embargo
Ir a EstadĂsticas
Este Ătem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/