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Oocytes use the plasminogen-plasmin system to remove supernumerary spermatozoa

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dc.contributor.advisorRomar Andrés, Raquel
dc.contributor.authorGrullón LA
dc.contributor.authorCoy Fuster, Pilar
dc.contributor.authorJíménez Movilla, María
dc.contributor.authorGarcía Vázquez, Francisco Alberto
dc.contributor.authorMondéjar Corbalán, Irene
dc.contributor.authorRomar Andrés, Raquel
dc.contributor.departmentFisiología
dc.date.accessioned2026-03-30T10:43:34Z
dc.date.available2026-03-30T10:43:34Z
dc.date.copyright©The Author 2012
dc.date.issued2012-05-03
dc.description.abstractbackground: The role of the plasminogen-plasmin (PLG-PLA) system in fertilization is unknown, although its dysfunction has been associated with subfertility in humans. We have recently detected and quantified plasminogen in the oviductal fluid of two mammals and showed a reduction in sperm penetration during IVF when plasminogen is present. The objective of this study was to describe the mechanism by which PLG-PLA system regulates sperm entry into the oocyte. methods and results: By combining biochemical, functional, electron microscopic, immunocytochemical and live cell imaging methods, we show here that (i) plasminogen is activated into the protease plasmin, by gamete interaction; (ii) urokinase-type and tissuetype plasminogen activators are present in oocytes, but they are not of cortical granule origin; (iii) sperm binding to oocytes triggers the releasing of plasminogen activators and (iv) the generated plasmin causes sperm detachment from the zona pellucida. conclusions: Our results describe a novel mechanism for the success or failure of fertilization in mammals, by which molecules present in the oviductal environment are activated by molecules originating within the gametes. We anticipate that therapeutic up- or down-regulation of this physiological mechanism may be used to help in conception or as a contraceptive tool. Since components of the PLG-PLA system are already available as drugs for heart attacks or cancer therapies, basic research on this novel function would be rapidly transferable for clinical application.
dc.formatapplication/pdf
dc.format.extent9
dc.identifier.citationHuman Reproduction, Vol.27, No.7 pp. 1985–1993, 2012
dc.identifier.doi10.1093/humrep/des146
dc.identifier.eissn1460-2350
dc.identifier.issn0268-1161
dc.identifier.urihttp://hdl.handle.net/10201/224201
dc.languageeng
dc.relationSpanish Ministry of Science and Innovation and FEDER, Grant AGL2009-12512-C02-01.
dc.relation.publisherversionhttps://academic.oup.com/humrep/article-abstract/27/7/1985/801445
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectPlasminogen
dc.subjectPlasmin
dc.subjectPlasminogen activators
dc.subjectSperm–zona pellucida binding
dc.subjectFertilization
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleOocytes use the plasminogen-plasmin system to remove supernumerary spermatozoa
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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