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The immunolocalization of HIF-2α, GLUT1 and CAIX in porcine oviduct during the estrous cycle

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dc.contributor.authorPárraga Ros, Ester
dc.contributor.authorLatorre Reviriego, Rafael Manuel
dc.contributor.authorAparicio Gozález, Mónica
dc.contributor.authorBoronat Belda, Talía
dc.contributor.authorLópez Albors, Octavio Miguel
dc.contributor.departmentAnatomía y Anatomía Patológica Comparada
dc.date.accessioned2025-12-23T14:37:52Z
dc.date.available2025-12-23T14:37:52Z
dc.date.copyright© 2022 The Authors
dc.date.issued2022-06-09
dc.description.abstractOxygen (O2) rates in the oviduct are essential to human and animal reproduction. These rates are regulated by the activity of hypoxia markers such as the hypoxia-inducible factors (HIFs), the glucose transporters (GLUT), and the carbonic anhydrase (CA). In the porcine model, scarce studies have been reported regarding these markers and their effects in reproduction are unknown. The objective was to characterize the immunolocalization of HIF-2α, GLUT1, and CAIX in porcine oviducts throughout the estrous cycle. Oviducts (ampulla and isthmus) of adult sows (n = 45) were collected for histological and immunohistochemical analysis with HIF-2α, GLUT1, and CAIX markers. The percentage of immunopositive area was quantified, and the differences among phases of the estrous cycle were analyzed (folicular, early luteal, and late luteal). The three markers showed epithelial presence mainly. Significantly lower expression of HIF-2α was found in the luteal phases, especially in the isthmus. GLUT1 expression did not change throughout the estrous cycle, but differences were found between the ampulla and isthmus. CAIX expression showed the highest, with a significant downward trend throughout estrous cycle. The ubiquitous expression of hypoxia markers shows the porcine oviduct physiology in relation to O2. The differential expression of HIF-2α, GLUT1, and CAIX in different subcompartments of the oviduct throughout the estrous cycle contributes to improve the knowledge of the cell physiology of the oviduct, which can be useful in fertilization studies.
dc.formatapplication/pdf
dc.format.extent11
dc.identifier.citationAnatomical Record. 2023;306:176–186.
dc.identifier.doi10.1002/ar.25014
dc.identifier.eissn1932-8494
dc.identifier.issn1932-8486
dc.identifier.urihttp://hdl.handle.net/10201/182369
dc.languageeng
dc.publisherWiley
dc.relationSin financiación externa a la Universidad
dc.relation.publisherversionhttps://anatomypubs.onlinelibrary.wiley.com/doi/10.1002/ar.25014
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHIF-2α
dc.subjectGLUT1
dc.subjectCAIX
dc.subjectOviduct
dc.subjectPorcine model
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleThe immunolocalization of HIF-2α, GLUT1 and CAIX in porcine oviduct during the estrous cycle
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication269b9699-1b31-4bb4-82ab-517084bcf737
relation.isAuthorOfPublicationd0c90149-4c92-4e44-b637-88131f331585
relation.isAuthorOfPublication39ee7277-9165-4e46-adc9-72006a802990
relation.isAuthorOfPublication.latestForDiscovery269b9699-1b31-4bb4-82ab-517084bcf737
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