Publication:
Human trophoblast cell during first trimester after IVF-ET differs from natural conceived pregnancy in development and function

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Yang-32-243-251-2017.pdf(1.82 MB)
Date
2017
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Authors
Yang, Rui ; Liu, Ying Ying ; Zhao, Liang ; Wang, Ying ; Li, Rong ; Liu, Ping ; Ma, Cai Hong ; Chen, Xin Na ; Qiao, Jie
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-787
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info:eu-repo/semantics/article
Description
Abstract
Objective. To explore the differences of the trophoblast cell function in first trimester between natural pregnancy and pregnancy after IVF-ET therapy. Methods. 102 cases with twin to singleton fetal reduction after IVF-ET treatment from July 2010 to August 2013 in Peking University Third Hospital were involved in analysis, and eight specimens were obtained from this group. 10 natural-pregnancy cases undergoing artificial abortion with unwanted pregnancy were recruited as control. Semi-quantitative immunohistochemical method was used to detect the expression of EGFR, Bcl-2, tubulin-α, metallothionein and AFP in villi in both groups. Results. Of the 102 cases, 14 cases (13.73%) were aborted. Preterm birth occurred in seven cases (7.86%). Low birth weight occurred in three patients (3.37%), and extremely low birth weight occurred in four cases (4.49%). The expression of EGFR, tubulin-α, Bcl-2, and metallothionein in the IVF-ET group was significantly lower than that in the control group (P<0.05). However, AFP expression was significantly higher in IVF-ET group than in control group (P<0.05). In IVF-ET group, the miscarriage case had weaker EGFR, tubulin-α, and metallothionein expression than full-term pregnancy; the early preterm labor case had weaker Bcl-2, tubulin-α, and metallothionein expression; and velamentous cord insertion case had weaker tubulin-α expression. Conclusions. The trophoblast cell function of IVF-ET group in first trimester is different from control group in proliferation, invasion, apoptosis and vascular development, and optimal pregnancy outcome depends on the self-healing balance of trophoblast cells.
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Cytotrophoblast cell , Syncytiotrophoblast cell , In vitro fertilization and embryo transfer , Natural pregnancy , Proteomic analysis
Citation
Histology and Histopathology, Vol.32, nº3, (2017)
URI
http://hdl.handle.net/10201/117047
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Histology and histopathology Vol.32, nº3 (2017)
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