Publication: Cells from the inner mass of blastocyst as a
source of neural derivates for differentiation studies
Authors
Álvarez, A. ; Gómez-Urquijo, S. ; Ramos, A. ; Hilario, E.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Our results show that cells derived from the
inner cell mass (ICM) show a clear tendency to
differentiate into the neural lineage, showing both cells
and structures in different degrees of differentiation.
Among the experimental paradigms used to learn about
neural differentiation, there have been several lines of
investigation on stem cells, including embryonic stem
(ES) cells isolated from the inner cell mass of embryo
and also stem cells derived from embryonic carcinoma
(EC). In this work, we have used a cellular line obtained
from the inner cell mass of a blastocyst. The cells were
cultured and after inoculated subcutaneously in syngenic
mice. The neural differentiation was predominant, and
could be observed both by morphological and
immunohistochemical methods. It was represented by
neural-tubes, neurons and glial cells, as expressed by the
presence of Microtubule-associated protein-2 (MAP-2)
and glial fibrilary acidic protein. Moreover, tyrosine
hydroxilase positive labelling was found in neuron-like
cells, which suggest the chatecolaminergic
differentiation. These results show that isolation of cells
from the inner mass of blastocyst represents an easy,
reproducible and cheap source of neural derivates
suitable for both in vivo and in vitro differentiation
studies.
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