Publication: Cardiovascular autonomic neuropathy in spontaneously diabetic rats with and without application of EGb 761
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Date
2010
Authors
Schneider, Rick ; Welt, Klaus ; Aust, Wolfram ; Kluge, Regina ; Löster, Heinz ; Fitzl, Günther
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Cardiovascular autonomic neuropathy causes
abnormalities in the diabetic heart with various clinical
sequelae, including exercise intolerance, arrhythmias
and painless myocardial infarction. Little is known about
(ultra)structural alterations of the myocardial nervous
network. On the assumption that this diabetes-specific
neuropathy develops due to permanently increased
oxidative stress by liberation of oxygen-free radicals,
adjuvant application of antioxidative therapeutics
appears promising in preventing or delaying long-term
diabetic complications. We have investigated the effects
of Ginkgo biloba extract (EGb 761), a radical scavenger,
against diabetes-induced myocardial nervous damage in
spontaneously diabetic BioBreeding/Ottawa Karlsburg
(BB/OK) rats. Morphological and morphometric
parameters were evaluated by electron microscopy. We
used immunohistochemistry to investigate protein
expression of protein gene product 9.5, S100 protein,
and thyroxin hydroxylase as a neuronal marker.
Alterations of cardiac sympathetic activity were
measured using the in vivo 123I-metaiodobenzylguanidine
imaging, and the immunofluorescent labeling
of beta1-adrenergic receptors and adenylate cyclase. Our
results revealed that A) Diabetes results in slight to
moderate ultrastructural alterations (hydrops,
disintegration of substructure) of autonomic nerve fibers
and related Schwann cells in untreated BB diabetic rats;
B) Cardiac sympathetic integrity and activity is impaired
due to alterations in the presynaptic nerve terminals and
the postsynaptic ß1-AR-AC coupling system; C) Pretreatment
of diabetic myocardium with EGb results in an
improvement of most of these parameters compared to
unprotected myocardium. In conclusion, EGb may act as
a potent therapeutic adjuvant in diabetics with respect to
cardiovascular autonomic neuropathy, which may
contribute to the prevention of late complications in
diabetes.
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