Publication: Effects of ethanol on the ultrastructure of the hamster femur
Authors
chen, H. ; Hayakawa, D. ; Emura, S. ; Ozawa, Y. ; Taguchi, H. ; Yano, R. ; Shoumura, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Several previous studies have indicated that
chronic ingestion of ethanol exerts harmful effects on
bones. However, few data are available concerning the
effects of ethanol on the ultrastructure of bone. To
further elucidate the effects of ethanol on bone, we
studied the morphology of femur in golden hamsters
after long-term treatment with ethanol. Six-week-old
male hamsters were divided into 4 groups. Ethanoltreated
animals were given ethanol at a concentration of
7% with food and water freely available, whereas the
pair-fed animals (weight-matched to ethanol hamsters)
had tap water available as the only drinking fluid. The
femur weight, blood ethanol and serum calcium
concentrations were determined after 3 and 5 months.
The bone mineral density (BMD) of the whole body was
measured before and after the experiment. Femurs of
both sides were dissected and processed for
morphometric measurement, light microscopy, scanning
and transmission electron microscopy. In the ethanoltreated
hamsters, BMD of the whole body and the
weight of femur tended to decrease when compared with
those of the controls. Light microscopy and scanning
electron microscopy showed that the trabecula in the
dista1 end of the femur from ethanol-treated hamsters
were thinner than those of the controls. We also
observed the disrupted swollen mitochondria of the
femoral osteoblasts and osteocytes in the ethanol-treated
hamsters. No significant difference in serum calcium
levels and femoral osteoclasts was found. These results
indicate that long-term treatment with ethanol results in
disruption of femoral osteoblasts and reduction of bone
mass in trabecular bone.
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