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Metabolic profile and glycemic response in fully-grown sows born using assisted reproductive technologies

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dc.contributor.authorCanovas, Sebastian
dc.contributor.authorHeras, Sonia
dc.contributor.authorQuintero-Moreno, Armando
dc.contributor.authorGadea, Joaquin
dc.contributor.authorCoy, Pilar
dc.contributor.authorRomar, Raquel
dc.contributor.authorRomero Aguirregomezcorta, Jon
dc.contributor.departmentFisiologíaes
dc.date.accessioned2025-09-04T07:15:19Z
dc.date.available2025-09-04T07:15:19Z
dc.date.copyright© 2024 The Authors
dc.date.issued2024-10-02
dc.description.abstractThe aim of the present work was to gain insight into the metabolism of pigs derived from assisted reproductive technologies during their adulthood. Approximately 4h after feeding, a blood sample was taken from 3.5 year old sows born by artificial insemination (AI group, n = 7) and transfer of in vitro produced embryos (IVP group, n = 11) to determine the physiological concentrations of the main biomarkers of carbohydrates (glucose and lactate), proteins (albumin, creatinine and urea) and lipids (cholesterol and triglycerides). Four weeks later, an oral glucose tolerance test (OGTT; 1.75g glucose/kg body weight) was performed after an overnight fast and 1h of water withdrawal. Blood samples were obtained prior (T = 0 min; fasting conditions) and 15, 30, 45, 60, 90, 120, 150, 180, 210 and 240 min after glucose intake. At each time point, glycemia was measured immediately using glucometer test strips, and serum was collected to determine the above metabolites along with insulin and glucagon. After OGTT, the area under the curve (AUC) between sampling times and homeostasis model assessment of insulin resistance (HOMA) indices were calculated. Under physiological conditions, the concentration of metabolites studied was similar between AI and IVP sows. In both groups, fasting decreased cholesterol and increased triglycerides and urea (P < 0.001). However, creatinine and lactate were similar in both groups under physiological and fasting conditions. The expected increase in albuminemia and decrease in glycaemia after fasting was only observed in IVP sows. OGTT revealed a different glucose curve pattern (monophasic in AI and biphasic in IVP group), a lower mean concentration of cholesterol, glucose, lactate, triglycerides in IVP compared to AI pigs (P < 0.01), and a higher mean concentration of albumin, creatinine and insulin in IVP compared to AI group (P < 0.05). On the contrary, no differences were found between groups for mean serum glucagon and urea levels, nor for glucose homeostasis indices HOMA-IR and HOMA-%B. The AUC differed between groups at several time points with larger AUC for creatinine, and smaller AUC for glucose, glucagon, and triglycerides, in IVP pigs than in AI pigs at 180–210 min (P < 0.05). In conclusion, under physiological conditions the metabolic profile of fully-grown AI and IVP sows is similar and within normal ranges. Glucose challenge revealed differences in metabolic and insulin responses between groups but with normal glucose tolerance in both cases.es
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dc.format.extent8es
dc.identifier.citationTheriogenology 230 (2024) 314–321
dc.identifier.doihttps://doi.org/10.1016/j.theriogenology.2024.10.002
dc.identifier.issnPrint: 0093-691X
dc.identifier.issnElectronic: 1879-3231
dc.identifier.urihttp://hdl.handle.net/10201/158143
dc.languageenges
dc.publisherElsevieres
dc.relationEsta publicación es parte del proyecto nacional de I+D+i PID2020-113366RB-I00, financiado por MCIN/ AEI/10.13039/501100011033. “FEDER Una manera de hacer Europa”. SH fue financiada por el programa Juan de la Cierva-Incorporación IJC2019-039404-I (MCIN/AEI/10.13039/501100011033) y AAQM por una ayuda “Maria Zambrano”, del Programa para la Recualificación del Sistema Universitario Español (CO-MZ-14).es
dc.relation.ispartofPhenotypART -- Phenotypic and genetic characterization of livestock animals derived from assisted reproductive technologieses
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0093691X24004047es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectInsulines
dc.subjectGlucagones
dc.subjectMetabolismes
dc.subjectPiges
dc.subjectAssisted reproductive technologieses
dc.titleMetabolic profile and glycemic response in fully-grown sows born using assisted reproductive technologieses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication3cc83bb3-8c44-471d-bc14-7d7dff677509
relation.isAuthorOfPublication.latestForDiscovery3cc83bb3-8c44-471d-bc14-7d7dff677509
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