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Mammalian spermatozoa and cumulus cells bind to a 3D model generated by recombinant zona pellucida protein-coated beads.

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dc.contributor.authorHamze Araujo, Julieta Gabriela
dc.contributor.authorCanha-Gouveia, Analuce
dc.contributor.authorAlgarra, Blanca
dc.contributor.authorGómez-Torres, María José
dc.contributor.authorRomar Andrés, Raquel
dc.contributor.authorJíménez Movilla, María
dc.contributor.authorOlivares Sánchez, María Concepción
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.contributor.departmentBiotecnología (Universidad de Alicante)
dc.date.accessioned2025-01-24T08:31:13Z
dc.date.available2025-01-24T08:31:13Z
dc.date.copyright© The Authors 2019
dc.date.issued2019-11-29
dc.description.abstractThe egg is a spherical cell encapsulated by the zona pellucida (ZP) which forms a filamentous matrix composed of several glycoproteins that mediate gamete recognition at fertilization. Studies on molecular mechanisms of sperm-egg binding are limited in many mammalian species by the scarcity of eggs, by ethical concerns in harvesting eggs, and by the high cost of producing genetically modified animals. To address these limitations, we have reproduced a three-dimensional (3D) model mimicking the oocyte’s shape, by means of magnetic sepharose beads coated with recombinant ZP glycoproteins (BZP) and cumulus cells. Three preparations composed of either ZP2 (C and N-termini; BZP2), ZP3 (BZP3) or ZP4 (BZP4) were obtained and characterized by protein SDS-PAGE, immunoblot and imaging with confocal and electron microscopy. The functionality of the model was validated by adhesion of cumulus cells, the ability of the glycoprotein-beads to support spermatozoa binding and induce acrosome exocytosis. Thus, our findings document that ZP-beads provide a novel 3D tool to investigate the role of specific proteins on egg-sperm interactions becoming a relevant tool as a diagnostic predictor of mammalian sperm function once transferred to the industry.es
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dc.format.extentpdfes
dc.format.extent12
dc.identifier.citationScientific Reports, 2019, Vol. 9(1) :17989
dc.identifier.doihttps://doi.org/10.1038/s41598-019-54501-7
dc.identifier.issnElectronic: 2045-2322
dc.identifier.urihttp://hdl.handle.net/10201/149240
dc.languageenges
dc.publisherSpringer Naturees
dc.relationSupported by Fundación Seneca-Agencia de Ciencia y Tecnología de la Región de Murcia “Ayudas a la realización de proyectos para el desarrollo de investigación científica y técnica por grupos competitivos 2018” (20887/PI/18), MINECO and FEDER (AGL2015 70159 P), Groups and Units of Scientific Excellence of the Region of Murcia (20040/GERM/16) and the AGL2015–66341‐R from the Ministry of Economy and Competitiveness (Spain).es
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-019-54501-7
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBiological models
dc.subjectMedical research
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleMammalian spermatozoa and cumulus cells bind to a 3D model generated by recombinant zona pellucida protein-coated beads.es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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relation.isAuthorOfPublication0c5bf0d3-e651-4120-8e9e-18633c9be135
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relation.isAuthorOfPublication.latestForDiscoveryadc79cbe-b88e-45e3-a7f8-0ea952bc67d0
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