HSP47 expression in the hamster Sertoli cell: An immunohistochemical study

Serrano-Sánchez, María Isabel; Ferrer, Concepción; Beltrán Frutos, Ester; Martínez Hernández,  Jesús; Pastor García, Luis Miguel; Seco Rovira, Vicente

Publication:
HSP47 expression in the hamster Sertoli cell: An immunohistochemical study

dc.contributor.author	Serrano-Sánchez, María Isabel
dc.contributor.author	Ferrer, Concepción
dc.contributor.author	Beltrán Frutos, Ester
dc.contributor.author	Martínez Hernández, Jesús
dc.contributor.author	Pastor García, Luis Miguel
dc.contributor.author	Seco Rovira, Vicente
dc.date.accessioned	2024-10-01T11:22:04Z
dc.date.available	2024-10-01T11:22:04Z
dc.date.issued	2024
dc.description.abstract	HSP47, a chaperone whose main function is the maturation of collagen molecules, is considered a marker of fibrotic diseases. Increased collagen synthesis in the testis has been associated with various pathologies leading to seminiferous tubule regression. Our aim was to study whether HSP47 is expressed in hamster Sertoli cells both in the adult and in two physiological situations of seminiferous tubule atrophy: irreversible testicular ageing and testicular regression due to short photoperiod (reversible). Eighteen animals were divided as follows: a group of 6 young animals aged 6 months, a group of 6 animals aged 24 months, which were exposed to a long photoperiod, and a final group of 6 young animals subjected to a short photoperiod. Testicular samples were fixed in methacarn and an immuno-histochemical technique was used to detect HSP47. A semiquantitative study of this protein expression was performed between tubular sections of aged animals with complete spermatogenesis and arrested spermatogenesis and tubular sections with arrest spermatogenesis of photoinhibited testes. Sertoli cells were positive for HSP47, the intensity being greater in tubular sections with arrested spermatogenesis in both aged and photoinhibited animals. Semiquantitative analysis corroborated this observation in the sense that the expression of this protein differed according to the functional state of the seminiferous tubules. Thus, the ratio of immunoreactivity was significantly higher in tubular sections with arrested spermatogenesis in aged animals compared with regressed animals, and in the latter compared with those whose tubular sections showed complete spermatogenesis. In conclusion, HSP47 expression in Sertoli cells was found for the first time in mammals. Moreover, increased expression seemed to be related to the degree of seminiferous atrophy epithelium and to the reversible or nonreversible physiological state of this epithelium.	es
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.citation	Histology and Histopathology, Vol.39, nº10, (2024)
dc.identifier.doi	https://doi.org/10.14670/HH-18-734
dc.identifier.issn	1699-5848
dc.identifier.issn	0213-3911
dc.identifier.uri	http://hdl.handle.net/10201/144457
dc.language	eng	es
dc.publisher	Universidad de Murcia. Departamento de Biología Celular e Histología	es
dc.relation	Sin financiación externa a la Universidad	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Sertoli cell	es
dc.subject	Syrian hamster	es
dc.subject	Testes	es
dc.subject	HSP47	es
dc.subject	Aging	es
dc.subject	Photoperiod	es
dc.subject.other	CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología	es
dc.title	HSP47 expression in the hamster Sertoli cell: An immunohistochemical study	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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