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Granulocyte-macrophage colony stimulating factor (GM-CSF) is fully expressed in the genital tract, seminal plasma and spermatozoa of male pigs

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dc.contributor.authorPadilla, Lorena
dc.contributor.authorBarranco, Isabel
dc.contributor.authorLucas, Xiomara
dc.contributor.authorRodríguez-Martínez, Heriberto
dc.contributor.authorRoca, Jordi
dc.contributor.authorParrilla, Inmaculada
dc.contributor.authorMartínez Hernández, Jesús
dc.contributor.authorPastor García, Luis Miguel
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-09-13T07:11:07Z
dc.date.available2024-09-13T07:11:07Z
dc.date.issued2020-08-07
dc.description© 2020 by the authors.This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Scientific Reports. To access the final edited and published work see https://doi.org/10.1038/s41598-020-70302-9
dc.description.abstractGranulocyte-macrophage colony stimulating factor (GM-CSF) is a pro-inflammatory cytokine identified in boar seminal plasma (SP) but until now unexplored in terms of place of production and its association to spermatozoa. This study aimed to explore these aspects by evaluating the presence of GM-CSF in porcine reproductive organs (testes, epididymis and accessory sex glands), SP and mature spermatozoa (from cauda epididymis and ejaculated) using Western blot (WB), immunohistochemistry and immunocytochemistry. Positive labelling was obtained in tissues, SP and spermatozoa. In reproductive organs, WB revealed three forms of GM-CSF with different glycosylation degrees (15, 31 and 40 kDa). In SP and epididymal fluid, the GM-CSF appeared only in its active form while in spermatozoa the GM-CSF form present varied among sperm sources. Non-viable spermatozoa showed more GM-CSF than viable spermatozoa (14.87 ± 1.98 RU vs. 7.25 ± 0.52 RU) of fluorescence intensity. In conclusion, GM-CSF is widely present in the reproductive tract of male pigs, attached to the spermatozoa already in the epididymis as well as verted to SP. Consequently, the GM-CSF ought to regulate male genital tract and sperm function as well as mediating initial inflammatory responses and further mediating later immune actions by the female to semen deposition.es
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dc.identifier.citationScientific Reports volume 10, Article number: 13360 (2020)
dc.identifier.doihttps://doi.org/10.1038/s41598-020-70302-9
dc.identifier.issnElectronic: 2045-2322
dc.identifier.urihttp://hdl.handle.net/10201/143916
dc.languageenges
dc.publisherNature Research
dc.relationThis project was supported by MINECO and FEDER [AGL2015-69738-R] Madrid (Spain), Seneca Foundation [19892/GERM/15] Murcia (Spain), Swedish Research Council FORMAS [2017-00946 and 2019-00288], Stockholm, Sweden. We also thank the MINECO (Madrid, Spain), for its grant-based support to L. Padilla and I. Barranco.es
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-020-70302-9es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleGranulocyte-macrophage colony stimulating factor (GM-CSF) is fully expressed in the genital tract, seminal plasma and spermatozoa of male pigses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicatione442a579-c2af-4fdd-8e39-00e5581604cb
relation.isAuthorOfPublication920d1ad2-1252-4ca8-9187-f3a351c89ce2
relation.isAuthorOfPublication.latestForDiscoverye442a579-c2af-4fdd-8e39-00e5581604cb
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