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Role of acupuncture in improving the outcome of sepsis-induced lung injury

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dc.contributor.authorLi, Peng
dc.contributor.authorLi, Fangfang
dc.contributor.authorChen, Si
dc.contributor.authorMa, Qiulei
dc.contributor.authorWang, Jie
dc.contributor.authorMa, Bingquan
dc.contributor.authorXu, Jin
dc.date.accessioned2025-02-19T17:07:51Z
dc.date.available2025-02-19T17:07:51Z
dc.date.issued2025
dc.description.abstractObjective. The purpose of this study was to investigate the effect of serum exosomes of mice after acupuncture (acu-exo) on acute lung injury (ALI) in sepsis in vitro and in vivo. Methods. Serum exosomes (acu-exo) of normal mice were prepared after acupuncture. Lipopolysaccharide (LPS) was used to establish the model of ALI in vivo and in vitro. Immunohistochemistry, western blot, and immunofluorescence were used to evaluate the mechanism of acu-exo on ALI. P2X7 knockout mice and P2X7 siRNA were used to verify the mechanism. Results. Compared with normal mice, serum exosomes were significantly increased in normal mice after acupuncture. The results showed that P2X7 was increased in the lung of septic mice as compared with the WT group. It was also found that the increase in NLRP3 and NF-κB was accompanied by the activation of P2X7. Increased P2X7 led to activation of the P2X7 receptor causing mitochondrial dysfunctions in lung tissue of septic mice. Knockout of P2X7 or silenced P2X7 markedly decreased NLRP3 and NF-κB and led to mitochondrial function recovery in lung tissue of sepsis. At the same time, acu-exo significantly restored the above changes in the lung tissue of septic mice. Conclusions. Inhibition of P2X7 led to mito-chondrial function recovery of lung tissue by inhibiting NLRP3 and NF-κB. At the same time, acu-exo could improve ALI by decreasing NLRP3 and NF-κB activationes
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dc.format.extent12es
dc.identifier.citationHistology and Histopathology Vol. 40, nº03 (2025)
dc.identifier.doihttps://doi.org/10.14670/HH-18-781
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/150703
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectExosomeses
dc.subjectP2X7es
dc.subjectSepsises
dc.subjectAcute lung injuryes
dc.subjectNOD-like receptor thermal protein domain associated protein 3es
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleRole of acupuncture in improving the outcome of sepsis-induced lung injuryes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.40, nº3 (2025)