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Transcription factors GATA4 and TBX5 promote cardiomyogenic differentiation of rat bone marrow mesenchymal stromal cells

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dc.contributor.authorChen, Wei
dc.contributor.authorZhang, Lei
dc.contributor.authorShao, Su-Xia
dc.contributor.authorWang, Hai-Ping
dc.contributor.authorCui, Shi-Jie
dc.contributor.authorZhang, Ya-Nan
dc.contributor.authorKong, Xiang-Zeng
dc.contributor.authorYin, Qing
dc.contributor.authorZhang, Jin-Ping
dc.date.accessioned2021-02-22T08:55:50Z
dc.date.available2021-02-22T08:55:50Z
dc.date.issued2015
dc.description.abstract. Bone marrow mesenchymal stromal cells (BMSCs) have potential applications in cell and gene therapies for cardiac disease. The cardiac-specific transcription factors GATA-binding protein 4 (GATA4) and T-Box protein 5 (TBX5) are considered to be pivotal in cardiogenesis. The aim of this study was to investigate the effects of GATA4 and TBX5 on cardiomyogenic differentiation of BMSCs. The BMSCs were initially isolated and identified. Vectors harboring cardiac transcription factor genes GATA4 and TBX5 or empty vectors were transferred into BMSCs. Cardiomyogenic cells differentiated from BMSCs were identified by expression of cardiac-specific markers including cardiac troponin T, connexin 43, β-myosin heavy chain, and myosin light chain-2 using immunocytochemical staining, western blotting, and quantitative real-time PCR. The ultrastructures of the differentiated cells were examined by transmission electron microscopy, which were similar to those of fetal cardiomyocytes. The differentiated cells exhibited L-type calcium current activities reflective of the electrophysiological characteristics of cardiomyocytes. These findings indicate that exogenous expression of cardiac-specific transcription factors GATA4 and TBX5 enhance cardiomyogenic differentiation of BMSCses
dc.formatapplication/pdfes
dc.format.extent12es
dc.identifier.citationHistology and Histopathology, vol. 30, nº 12, (2015)
dc.identifier.doi0.14670/HH-11-644
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/103642
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectMesenchymal stromal cellses
dc.subjectDifferentiationes
dc.subjectGATA-binding protein 4es
dc.subjectT-Box protein 5es
dc.subjectCardiomyocyteses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleTranscription factors GATA4 and TBX5 promote cardiomyogenic differentiation of rat bone marrow mesenchymal stromal cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30,nº12 (2015)