Publication: Metalloproteinase expression by control and telomerase
immortalized human endometrial endothelial cells
Authors
Krikun, G. ; Mor, G. ; Huang, J. ; Schatz, F. ; Lockwood, C.J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Vascular endothelial cells play a critical role
in the maintenance of endometrial homeostasis. Indeed
many pathological conditions causing abnormal
endometrial bleeding including progestin only
contraception, hormone replacement therapy,
endometrial polyps, myomas, hyperplasia and cancer are
associated with aberrant angiogenesis. Critical to the
process of angiogenesis is the breakdown of the
surrounding tissues by matrix metalloproteases (MMPs).
In addition to the cells surrounding the endometrial
endothelial cells, the endothelial cells themselves
produce their own panel of MMPs. We now characterize
the specific MMPs that are expressed by endothelial
cells derived from human endometrium. These include
MMP-1, MMP-2 and MMP-10 but not MMP-3. In
addition, in order to successfully carry out consistent,
homogeneous and sufficient numbers of studies we
investigated the in vitro expression of the MMPs with
both freshly isolated, early passaged endometrial
endothelial cells (HEECs) as well as with newly
telomerase immortalized HEECs (T-HEECs). The latter
were karyotypically normal and expressed classic
endothelial cell endpoints such as tubulogenesis on
matrigel and expression of the endothelial cell markers
CD-31 (PECAM), von Willebrand’s factor, and the Tie-2
receptors. The levels of MMP expression as well as that
of the metalloprotease inhibitors TIMP-1 and TIMP-2
were similar in parent and immortalized endothelial
cells.
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