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Bone regeneration in critical-sized mandibular symphysis defects using bioceramics with or without bone marrow mesenchymal stem cells in healthy, diabetic, osteoporotic, and diabetic-osteoporotic rats

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dc.contributor.authorCamacho Alonso, Fabio
dc.contributor.authorTudela Mulero, M.R.
dc.contributor.authorPérez-Sayáns, M.
dc.contributor.authorMercado Díaz, A.M.
dc.contributor.authorNavarro Cámara, José Antonio
dc.contributor.authorBuendía Marín, Antonio Julián
dc.contributor.departmentDermatología, Estomatología, Radiología y Medicina Física
dc.date.accessioned2024-01-31T18:34:36Z
dc.date.available2024-01-31T18:34:36Z
dc.date.copyright© 2022
dc.date.issued2022
dc.description.abstractObjectives: To compare new bone formation in mandibular critical-sized bone defects (CSBDs) in healthy, diabetic, osteoporotic, and diabetic-osteoporotic rats filled with bioceramics (BCs) with or without bone marrow mesenchymal stem cells (BMSCs). Methods: A total of 64 adult female Sprague-Dawley rats were randomized into four groups (n = 16 per group): Group 1 healthy, Group 2 diabetic, Group 3 osteoporotic, and Group 4 diabetic-osteoporotic rats. Streptozotocin was used to induce type 1 diabetes in Group 2 and 4, while bilateral ovariectomy was used to induce osteoporosis in Group 3 and 4. The central portion of the rat mandibular symphysis was used as a physiological CSBD. In each group, eight defects were filled with BC (hydroxypatatite 60% and β-tricalcium phosphate 40%) alone and eight with BMSCs cultured on BC. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2). Results: In all groups (healthy, diabetics, osteoporotics, and diabetics-osteoporotics), the CSBDs filled with BC + BMSCs showed greater radiological bone union, BMD, histological bone union, and more VEGF and BMP-2 positivity, in comparison with CSBDs treated with BC alone (at 4 and 8 weeks).es
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dc.format.extent18es
dc.identifier.doihttps://doi.org/10.1016/j.dental.2022.06.019
dc.identifier.issn0109-5641
dc.identifier.urihttp://hdl.handle.net/10201/138326
dc.languageenges
dc.publisherElsevier SCI LTDes
dc.relationSin financiación externa a la Universidades
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBioceramicses
dc.subjectBone marrow mesenchymal stem cellses
dc.subjectMandibular symphysises
dc.subjectDiabeteses
dc.subjectOsteoporosises
dc.subject.otherCDU::5 - Ciencias puras y naturaleses
dc.titleBone regeneration in critical-sized mandibular symphysis defects using bioceramics with or without bone marrow mesenchymal stem cells in healthy, diabetic, osteoporotic, and diabetic-osteoporotic ratses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication41991b8b-3a95-46de-ab2a-b7968ee121e6
relation.isAuthorOfPublicationc084ce85-206c-418d-8cec-7f85a0f34dc0
relation.isAuthorOfPublication.latestForDiscovery41991b8b-3a95-46de-ab2a-b7968ee121e6
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