Publication: Distribution of bile acid receptor TGR5 in the gastrointestinal tract of dogs
Authors
Giaretta, Paula R. ; Suchodolski, Jan S. ; Blick, Anna K. ; Steiner, Joerg M. ; Lidbury, Jonathan A. ; Rech, Raquel R.
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
DOI: 10.14670/HH-18-025
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Description
Abstract
Takeda-G-protein-receptor-5 (TGR5) is a
receptor for bile acids and its expression has been
described in a variety of tissues and species.
Characterization of TGR5 distribution and function has
been investigated in drug discovery for the treatment of
metabolic diseases in humans. Because dogs are one of
the species used in biomedical research and share some
similarities with human gastrointestinal diseases, the
objective of this study was to characterize the
distribution of TGR5 receptor in the canine species. This
study characterizes the distribution of TGR5 receptor in
the gastrointestinal tract, liver, gallbladder, and pancreas
of 8 dogs. The distribution of TGR5 antigen and mRNA
expression was investigated using immunohistochemistry and RNA in situ hybridization, respectively.
TGR5 immunolabeling was located in the cell
membrane or in the cell membrane and cytoplasm.
TGR5 immunolabeling was broadly distributed in
macrophages, endothelial cells, ganglion cells, and
leiomyocytes throughout all the examined tissues.
Epithelial cells from tongue, stomach to rectum, as well
as from gallbladder, biliary and pancreatic ducts
demonstrated TGR5 immunolabeling. In endocrine cells,
TGR5 immunolabeling was observed in intestinal
enteroendocrine cells and islets of Langerhans in the
pancreas. The hepatocytes had a unique pattern of
immunolabeling located on the canalicular surface of the
cell membrane. TGR5 mRNA expression was located
mainly in the nucleus and the only negative cells
throughout all examined tissues were striated muscle
from tongue and esophagus, muscularis mucosae,
esophageal glands, and hepatic sinusoids. These findings
indicate that the bile acid receptor TGR5 is ubiquitously
distributed in the canine gastrointestinal tract.
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Citation
Histology and Histopathology, Vol.34, nÂş1, (2019)
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