Extension of life-span using a RNAi model and in vivo antioxidant effect of Opuntia fruit extracts and pure betalains in Caenorhabditis elegans

Guerrero Rubio, M. Alejandra; Hernández García, Samanta; Gandía Herrero, Fernando; García Carmona, Francisco

Publication:
Extension of life-span using a RNAi model and in vivo antioxidant effect of Opuntia fruit extracts and pure betalains in Caenorhabditis elegans

dc.contributor.author	Guerrero Rubio, M. Alejandra
dc.contributor.author	Hernández García, Samanta
dc.contributor.author	Gandía Herrero, Fernando
dc.contributor.author	García Carmona, Francisco
dc.contributor.department	Bioquímica y Biología Molecular A	es
dc.date.accessioned	2025-06-06T10:15:33Z
dc.date.available	2025-06-06T10:15:33Z
dc.date.issued	2018-09-11
dc.description	© 2018 The Authors. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Submitted, Accepted, Published Manuscript, version of a Published Work that appeared in final form in Food Chemistry. To access the final edited and published work see https://doi.org/10.1016/j.foodchem.2018.09.067	es
dc.description.abstract	Betalains are nitrogenous plant pigments known for their high antioxidant capacity. For the first time, this antioxidant nature has been studied in an in vivo system using the model organism Caenorhabditis elegans. The oxidative stress caused in the fluorescent strain TJ375 (hsp-16.2::GFP) was reversed by the presence of both natural and semi-synthetic betalains, with an ED50 value around 25 μM for betacyanins and up to 10 μM for betaxanthins, with indicaxanthin, the major pigments in prickly pear fruits, as the most effective betalain. The effect of model betalains on the lifespan of the wild-type N2 strain was carefully studied using the automatic platform “Lifespan Machine”. In a search for different approaches to suppress progeny, pop-1 RNAi was used to avoid FUdR use. The presence of betalains in the medium, both as pure compounds and as enriched Opuntia extracts significantly increased the lifespan of C. elegans	es
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.citation	Food Chemistry, 2019, Vol. 274, pp. 840-847
dc.identifier.doi	https://doi.org/10.1016/j.foodchem.2018.09.067
dc.identifier.issn	Print: 0308-8146
dc.identifier.issn	Electronic: 1873-7072
dc.identifier.uri	http://hdl.handle.net/10201/155720
dc.language	eng	es
dc.publisher	Elsevier
dc.relation	This work was supported by the Spanish Ministry of Economy and Competitiveness (MEC-FEDER) (AGL2014-57431 and AGL2017-86526) and by the “Programa de Ayudas a Grupos de Excelencia de la Región de Murcia, Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia” (Project 19893/GERM/15). M.A.G.-R. holds a contract financed by MEC-FEDER (Spain). S.H.G. holds a contract financed by Fundación Séneca (Spain).	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0308814618316406
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	C. elegans	es
dc.subject	Lifespan	es
dc.subject	Antioxidant	es
dc.subject	In vivo	es
dc.subject	Green fluorescent protein	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica	es
dc.title	Extension of life-span using a RNAi model and in vivo antioxidant effect of Opuntia fruit extracts and pure betalains in Caenorhabditis elegans	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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