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Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation

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Contreras-Aguilar et al. - 2017 - Detection and measurement of alpha- amylase in canine saliva and changes after an experimentally induc.pdf(803.45 KB)
Date
2017-08-22
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Authors
Contreras Aguilar, María Dolores ; Tecles Vicente, Fernando ; Martínez Subiela, Silvia ; Escribano Tortosa, Damián ; Bernal Gambín, Luis Jesús ; Cerón Madrigal, José Joaquín
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Publisher
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Medicina y Cirugía Animal Department of Food and Animal Science, School of Veterinary Medicine, Universitat Autònoma de Barcelona
DOI
https://10.1186/s12917-017-1191-4
https://doi.org/10.1186/s12917-017-1191-4
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info:eu-repo/semantics/article
Description
©2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published version of a Published Work that appeared in final form in BMC Veterinary Research. To access the final edited and published work see https://doi.org/10.1186/s12917-017-1191-4
Abstract
Background: Salivary alpha-amylase (sAA) is considered a biomarker of sympathetic activation in humans, but there is controversy regarding the existence of sAA in dogs. The hypothesis of this study was that sAA exists in dogs and it could change in situations of sympathetic stimulation. Therefore, the aims of this study were: 1) to demonstrate the presence of alpha-amylase in saliva of dogs by Western-Blot, 2) to validate an spectrophotometric method for the measurement of sAA activity and 3) to evaluate the possible changes in sAA activity after the induction of an ejaculation in dogs which is known to produce a sympathetic activation. Results: Western-Blot demonstrated a band in dog saliva specimens between 60 kDa and 50 kDa, similar to purified sAA. The spectrophotometric assay validated showed an adequate inter- and intra-assay precision, and a high correlation coefficient (r = 0.999) in the linearity under dilution study. sAA median activity significantly increased just after ejaculation compared with just before the ejaculation (2.06-fold, P =0.005). Conclusions: This study demonstrated the existence of alpha-amylase in saliva of dogs and that this enzyme can be measured by a spectrophotometric assay. In addition, results showed that sAA increase after a sympathetic activation and could be potentially used as non-invasive biomarker of sympathetic activity in this species.
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Salivary alpha-amylase , Dog , Saliva , Sympathetic activation
Citation
BMC Veterinary Research, 13, 266
URI
http://hdl.handle.net/10201/138117
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