Publication: lmmunohistochemical and in situ hybridization studies of choline acetyltransferase in large motor neurons of the human spinal cord
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Date
2000
Authors
Muroishi, Y. ; Kasashima, S. ; Nakanishi, I. ; Oda, Y.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The localization of choline acetyltransferase
(ChAT) protein and mRNA was investigated in large
motor neurons of the lumbar spinal cord of 10 autopsied
individuals without neurological diseases, by
immunohistochemistry and in situ hybridization. In the
immunohistochemistry using 20 serial tissue sections
with a total thickness of 80 pm, about 58-85% (average
67%) of the large motor neurons (30 pm and more in
somal minimal diameter) in the ventral horn were
stained with the anti-human ChAT antibody. In the
positive neurons, most immunoreactive products were
observed focally in the perikarya. Occasionally, the
perikarya of some neurons were stained diffusely. In situ
hybridization with a single 4 pm-thick tissue section
showed that almost all large motor neurons had positive
signals (93-loo%, average 98%), which were
distributed diffusely in the perikarya. The positivity rate
in the in situ hybridization was higher than that in the
immunohistochemistry for all 10 cases. These results
indicate that ChAT mRNA is transcribed in almost all
large motor neurons in the ventral horn of the human
spinal cord, but ChAT protein cannot always be detected
in the cytoplasm by immunohistochemistry.
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