Publication: First description of betalains biosynthesis in an aquatic organism: characterization of 4,5-DOPA-extradiol-dioxygenase activity in the cyanobacteria Anabaena cylindrica
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Date
2020-08-06
Authors
Guerrero Rubio, M. Alejandra ; Gandía Herrero, Fernando ; García Carmona, Francisco
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Publisher
Wiley
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DOI
https://doi.org/10.1111/1751-7915.13641
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info:eu-repo/semantics/article
Description
© 2020 The Authors. This manuscript version is made available under the CC-BY-NC 4.0 license http://creativecommons.org/licenses/by-nc/4.0/
This document is the Published version of a Published Work that
appeared in final form in Microbial Biotechnology. To access the final edited and published work see https://doi.org/10.1111/1751-7915.13641
Abstract
The biosynthesis of betalamic acid, the structuralunit of pigments betalains, is performed by enzymeswith 4,5-DOPA-extradiol-dioxygenase activity. Theseenzymes were believed to be limited to plants of the order Caryophyllales and to some fungi. However,the discovery of Gluconacetobacter diazotrophicusas the first betalain-forming bacterium opened a new field in the search for novel biological systems able to produce betalains. This paper describes molecular and functional characterization of a novel dioxyge-nase enzyme from the aquatic cyanobacterium Ana-baena cylindrica. The enzyme was found to be a homodimer of a polypeptide of 17.8 kDa that, oppo-site to previous related enzymes, showed a strong inhibition by excess of the precursor L-DOPA. How-ever, its heterologous expression has allowed detecting the formation of the main compounds in the biosynthetic pathway of betalains. In addition,phylogenetic analysis has shown that this enzyme is not close related to enzymes from plants, fungi orproteobacteria such as G. diazotrophicus. The pres-ence of enzymes that produce these health-promot-ing compounds is more diverse than expected. The discovery of this novel dioxygenase in the phylumcyanobacteria expands the presence of betalamicacid-forming enzymes in organisms of different nat-ure with no apparent relationship among them
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Citation
Microbial Biotechnology, 2020, Vol. 13, Issue 6, pp. 1948-1959
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