Publication: ERK1/2, JNK and STAT3 activation and correlation with tumor differentiation in oral SCC
Authors
Gkouveris, I. ; Nikitakis, N. ; Avgoustidis, D. ; Karanikou, M. ; Rassidakis, G. ; Sklavounou, A.
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-868
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info:eu-repo/semantics/article
Description
Abstract
Signal transducer and activator of
transcription 3 (STAT3) and mitogen activated protein
kinases (MAPKs), including ERK and JNK, have been
implicated in oral squamous cell carcinoma (OSCC)
development and progression. Our purpose was to
evaluate the levels of activated STAT3, ERK1/2 and
JNK by immunohistochemistry in OSCC and to
investigate possible correlations of these molecules with
each other as well as with the degree of tumor differentiation.
Immunohistochemical assessment of the phosphorylated levels of STAT3(tyrosine/ serine), ERK1/2 and
JNK was performed in 60 OSCC, including well,
moderately and poorly differentiated tumors. Semiquantitative scoring system was used, by calculating
intensity of immunostaining, percentage of positive cells
and combined scores. Statistics included Fisher’s test,
Student’s T-Test and Kruskal-Wallis analysis,
Spearman’s correlation coefficient and multivariate
logistic regression analyses.
Immunohistochemical levels of both pSTAT3(tyr)
and pERK1/2 showed statistically significant differences
between well and poorly differentiated tumors with the
latter receiving higher mean percentage, intensity and
total scores. On the other hand, pJNK showed
statistically significantly higher intensity levels in
moderately compared to poorly differentiated tumors.
pSTAT3(ser) immunoexpression did not appear to
correlate with tumor differentiation. Between different
molecules, more pronounced, pERK1/2 levels exhibited
statistically significant positive correlation with
pSTAT3(ser), pSTAT3(tyr) and pJNK expression.
ERK1/2 and STAT3 activation (as assessed by
tyrosine but not serine phosphorylation) could contribute
to a less differentiated phenotype in OSCC, while JNK
activation may have an opposite, although possibly less
pronounced, effect. Positive correlations between MAPK
and STAT3 levels may indicate a direct crosstalk and/or
regulation by common upstream pathways.
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Citation
Histology and Histopathology, Vol.32, nº10, (2017)
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