Publication: Expression of p53, p21 waf l , bcl-2, bax, Rb and Ki67 proteins in Hodgkin's lymphomas
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Date
2000
Authors
Kanavaros, Panagiotis ; Stefanaki, K. ; Vlachonikolis, J. ; Eliopoulos, G. ; Kakolyris, S. ; Rontogianni, D. ; Gorgoulis, V. ; Georgoulias, V.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim was to investigate the combined
immunoexpression of p53, p21, bcl-2, bax, Rb and Ki67
proteins in Hodgkin's lymphomas (HL) and correlate
expression patterns with the histotype and the Epstein-
Barr Virus (EBV) status. Paraffin-sections from 56 cases
of HL (18 nodular sclerosis and 38 mixed cellularity)
and from ten "reactive" lymph nodes were investigated.
P53, p21, bcl-2, bax, Rb and Ki67 proteins were
detected in Hodgkin and Reed-Sternberg (HRS) cells in
35156,56156,24156, 23156,56156 and 56/56 cases of HL,
respectively. No correlation was found between the
expression of each protein and the EBV status or the
histotype of HL. Comparison between p53 and p21
staining revealed two patterns: a) p53+/p21+ (35 cases);
and b) p53-/p21+ (21 cases). The pattern p53+/p21+
suggests wild type p53 protein able to induce the
expression of p21 while the p53-/p21+ pattern suggests
p53-independent p21 expression. These results are
consistent with the interpretation that inactivating p53
gene mutations may be rare in HL. Comparison between
bcl-2 and bax staining showed a statistically significant
relationship (p<0.001) for coexpression (19 cases) or
absence of expression of both proteins (28 cases) in HRS
cells. In contrast, bax expression was observed in most
lymphoid cells in all "reactive" lymph nodes. Since the
proapoptotic bax protein may act as tumour suppressor it
is possible that the absence of this ,protein in HRS cells
in a substantial proportion of HL may confer growth
advantage and play a role in their pathogenesis. This
could suggest bax gene alterations in some HL since in
other studies acute lymphoblastic leukaemia cell lines
demonstrate bax gene mutations with loss of bax
immunoexpression. Another possibility is that reduced
bax expression may be due to post transcriptional
regulation, as was described in lymphoma cell lines.
Comparison between Rb and Ki67 staining disclosed
two main deviations from the normal parallel
relationship in reactive lymph nodes: a) 2 cases with low
Offprint requests to: Prof. V. Georgoulias, Deparrnent of Oncology,
University Hospital of Heraklion. 71 100 Heraklion, Crete. Hellas,
Greece. Fax: 0030 811392802
Rb and high Ki67 expression possibly reflecting loss of
Rb expression due to chromosome loss or to other
abnormalities in the structure or the expression of Rb
gene; and b) 9 cases with high RB and low Ki67 possible
reflecting an attempt of Rb protein in excess to induce
cell cycle arrest. Taken together, our findings provide
combined immunohistological evidence for deregulated
expression of cell-cycle and apoptosis-related proteins,
that may play a role in the pathogenesis of HL.
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