Publication:
Use of a crude extract or purified antigen from first-instar cattle grubs, Hypoderma lineatum, for the detection of anti-Hypoderma antibodies in free-ranging cervids from southern Spain

Simple item page
dc.contributor.authorPanadero, Rosario
dc.contributor.authorMartínez-Carrasco Pleite, Carlos
dc.contributor.authorLeón Vizcaíno, Luis
dc.contributor.authorLópez, C.
dc.contributor.authorDíez Paños, P.
dc.contributor.authorMorrondo, M. P.
dc.contributor.authorAlonso de Vega, Francisco-Domingo
dc.contributor.departmentSanidad Animal
dc.contributor.otherFacultades de la UMU::Facultad de Veterinaria
dc.date.accessioned2026-01-07T09:35:57Z
dc.date.available2026-01-07T09:35:57Z
dc.date.copyright© 2010 The Authors. Medical and Veterinary Entomology © 2010 The Royal Entomological Society
dc.date.issued2010-11-08
dc.description.abstractDuring the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme-linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.
dc.formatapplication/pdf
dc.format.extent10
dc.identifier.citationMedical and Veterinary Entomology, 2010, Vol. 24, pp. 418- 424
dc.identifier.doi https://doi.org/10.1111/j.1365-2915.2010.00892.x
dc.identifier.eissn1365-2915
dc.identifier.issn0269-283X
dc.identifier.urihttp://hdl.handle.net/10201/183489
dc.languageeng
dc.publisherWiley
dc.relationWe are grateful to the Food and Agrarian National Technol­ogy Institute (MEC), Madrid, Spain for concessions under projects AGL-2002-02916 and AGL-2004-01827. We also thank Dr Jesús M. Pérez for kindJy providing us with climatic data for Sierras de Cazorla, Segura and Las Villas Natural Park.
dc.relation.publisherversionhttps://resjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2915.2010.00892.x
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectHypoderma antibodies
dc.subjectFree ranging cervids
dc.subjectSouthern Spain
dc.subjectHypoderma lineatum
dc.subject.odsObjetivo 15: Bosques, desertificación y diversidad biológica
dc.subject.odsObjetivo 3: Salud
dc.titleUse of a crude extract or purified antigen from first-instar cattle grubs, Hypoderma lineatum, for the detection of anti-Hypoderma antibodies in free-ranging cervids from southern Spain
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublication74bbaf1b-2a73-4ec8-b1c6-429311688d3b
relation.isAuthorOfPublication1876da0c-4165-402e-b95e-3da842640ad7
relation.isAuthorOfPublication.latestForDiscovery74bbaf1b-2a73-4ec8-b1c6-429311688d3b
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Hypoderma_antibodies_cervids_2010.pdf
Size:
1.95 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.37 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos