Publication: Three-dimensional architecture of the connective tissue core and surface structures of the lingual papillae in the rabbit
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Date
2002
Authors
Silva, M.C.P. ; Watanabe, I. ; Kronka, M.C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Three-dimensional characteristics of the
epithelial cell layer and connective tissue interface of the
tongue were studied using scanning electron microscopy.
In this study, the fragments of tongue were fi xed in
m o d i fied Karnov s ky ’s fi x a t ive solution. Subsequently,
the specimens were treated with 10% NaOH solution for
4-7 days at room temperature and postfixed in 1% OsO4
in 0.1M phosphate buffer (pH 7.4) for 2 hours at 4 °C.
T h ey were dehydrated through a graded ethanol series,
and critical-point dried with CO2. The specimens were
coated with gold and observed in a scanning electron
microscope, JEOL JSM-6100. The results show e d
numerous papillae on the dorsal surface of the tongue
divided into four groups (filiform, fungiform, foliate and
vallate papillae). Filiform papillae are conically shaped;
fungiform papillae have an irregular round surfa c e ;
foliate papillae are oval in shape and have some parallel
projections; and vallate papillae are located in the
posterior part of the tongue and have a depression
around the center. After the treatment with 10% NaOH
solution, the original arrangements of connective
papillae could be seen. This characteristic threedimensional
distribution of the collagen fiber bundles is
typical for each superficial papillae depending on
whether it is filiform, fungiform, foliate or vallate.
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