Publication: Overexpression of hsa_circ_0094742
inhibits IL-1β-induced decline in CHON-001
cell viability by targeting microRNA-127-5p
Authors
Sun, Mingqi ; Yang, Junli ; Jiang, Dianming ; Bao, Guoyu
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-325
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info:eu-repo/semantics/article
Description
Abstract
Osteoarthritis (OA) is a public health
problem that affects 240 million people globally;
however, the current treatment options for OA are not
effective. Therefore, there is still an urgent need to
identify novel strategies to reduce the incidence and
progression of OA. The circular RNA hsa_circ_0094742
was reported to be downregulated in patients with OA.
However, the underlying mechanism remains unclear.
The levels of hsa_circ_0094742 in CHON-001 were
detected by reverse transcription quantitative polymerase
chain reaction. Moreover, Cell Counting Kit-8 assay and
Ki67 staining were used to determine the cell viability.
The protein expression of biomarkers was detected by
western blot analysis. In addition, the putative
downstream target of hsa_circ_0094742 was predicted
using the Circinteractome and TargetScan online
databases. The putative targeting relationship was
verified by dual luciferase reporter assay and
fluorescence in situ hybridization. Next, cell apoptosis
was determined by Annexin V/PI staining.
hsa_circ_0094742 overexpression (OE) inhibited
interleukin (IL)-1β-induced decline in the viability of
CHON-001 cells and primary human chondrocytes.
Furthermore, IL-1β-induced alterations in aggrecan,
matrix metallopeptidase 13, X-linked inhibitor of
apoptosis protein (XIAP), Bax and active caspase 3 were
reversed by hsa_circ_0094742 OE. Luciferase reporter
assay indicated that miR-127-5p was the downstream
target of hsa_circ_0094742, and latexin was the target of
miR-127-5p. hsa_circ_0094742 OE inhibited IL-1βinduced decline in CHON-001 cell viability by targeting
miRNA-127-5p. The findings of the present study
revealed the biological rational of the use of
hsa_circ_0094742 OE as an anti-IL-1β effector in
human chondrocytes. These findings may prompt further
research on hsa_circ_0094742 as a potent circRNA
target for the treatment of OA.
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Citation
Histology and Histopathology Vol. 36, nº2 (2021)
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