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Immunohistochemical study of the apoptosis process in epidermal epithelial cells of rats under a physiological condition

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dc.contributor.authorUdayanga, K.G.S
dc.contributor.authorMiyata, H.
dc.contributor.authorYokoo, Y.
dc.contributor.authorQi, W.M.
dc.contributor.authorTakahara, E.
dc.contributor.authorMantani, Y.
dc.contributor.authorYokoyama, T.
dc.contributor.authorHoshi, N.
dc.contributor.authorKitagawa, Hiroshi
dc.date.accessioned2016-04-25T17:40:27Z
dc.date.available2016-04-25T17:40:27Z
dc.date.issued2011
dc.description.abstractEpidermal homeostasis is maintained by both epithelial proliferation in the stratum basale (SB) and the apoptosis of epithelial cells under physiological conditions. In this study, the induction and regulation mechanisms of epidermal apoptosis were immunohistochemically investigated in the epidermis from Wistar rat’s palm and foot pad by using several apoptotic related proteins under a physiological condition. The results showed that Fas and Fas-L were expressed in cellular membranes of the stratum spinosum (SS), whereas TNF-R1 did not show any membranous expression in any epidermal layers. TNF-α was not observed in the epidermis. Caspase-10, cleaved caspase-3 and DNase-1 were found in the epithelial cytoplasms from the SS to stratum granulosum (SG), whereas caspase-8 was not detected in the epidermis. XIAP and Bak were found in the cytoplasm from the SS to SG, and the intensity of Bak-positivity was stronger in the SG than the SS, whereas Bid, Apaf-1 and cleaved caspase-9 were restricted in the SG. Homogenous cytoplasmic immunoreactivity of Bcl-2 was found in the SB and the intensity was gradually decreased from the SB to the SG. The granular-cytoplasmic immunopositivity of cytochrome C gradually altered into homogenous cytoplasmic expression in the upper half of the SG. Single-stranded DNA was rarely detected in the upper portion of the SG. These results suggest that epidermal apoptosis is induced by the interaction between Fas and Fas-L and the activation of caspase-10, and might initially proceed through a mitochondrialindependent pathway, and that a mitochondrialdependent pathway finally accelerated under physiological conditions.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/49322
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Murcia: Universidad de Murcia, Departamento de Biología Celular e Histologíaes
dc.relation.ispartofHistology and histopathology, Vol. 26, nº 7 (2011)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectEpidermises
dc.subjectImmunohistochemistryes
dc.subject.other636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.titleImmunohistochemical study of the apoptosis process in epidermal epithelial cells of rats under a physiological conditiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.26, nº7 (2011)