Publication: Lysimachia christinae Hance aqueous extract ameliorates renal injury in kidney stone rats and calcium oxalate crystal-induced oxidative stress in HK-2 cells via inhibiting the PI3K/Akt/mTOR pathway
Authors
Shengni Lv ; Wangen Wang ; Liangrong Zhu ; Luning Lin ; Xintian Zheng
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Publisher
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DOI
https://doi.org/10.14670/HH-18-964
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info:eu-repo/semantics/article
Description
Abstract
Objectives. Kidney stones are a frequent
urinary system disorder. Lysimachia christinae Hance is
an accepted herb in traditional Chinese medicine for
treating kidney stones. However, the effects and
mechanisms of Lysimachia christinae Hance aqueous
extract (LCH) are yet to be elucidated.
Methods. The function of the aqueous extract of
LCH was assessed using kidney stone rat models
induced by 1% ethylene glycol+2% NH4Cl.
Additionally, an in vitro model of human renal tubular
epithelial cells (HK-2) treated with calcium oxalate was
used.
Results. Resultantly, the treatment of aqueous extract
of LCH at different concentrations or LCH+LY294002
(PI3K-specific inhibitor) showed significant improve
ment in inorganic ions and renal pathological injury in
nephrolithiasis rats. Besides, consistent with the in vivo
assay, LCH-containing serum increased cell viability and
inhibited oxidative stress and deposition of Ca2+ in HK
2 cells, while the influences of LCH-containing serum
were attenuated. Mechanistically, the aqueous extract of
LCH and LCH-containing serum also promoted Nrf2
and HO-1 levels and inhibited the phosphorylated
expression levels of PI3K, AKT, and mTOR.
Conclusion. This study shows that LCH ameliorates
the kidney damage in kidney stone rats and HK-2 cells.
The mechanism of LCH in treating kidney stones is
related to the activation of the Nrf2/HO-1 axis and the
inhibition of the PI3K/Akt/mTOR pathway.
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