Publication: Seasonality and freezability vs
routine parameters in stallion semen
Authors
Rodríguez, H. ; Bustos-Obregón, E.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The fertilizing ability of stallion semen was
analyzed using fresh and frozen samples, obtained
before (June-July) or during (October-November) the
breeding season. Thirty ejaculates obtained from 4
stallions, were used. The analysis comprises routine
seminogram; ATP concentration (Comhaire et al., 1983);
subjective and objective motility and sperm velocity
(Makler, 1980). Freezing was done following the
technique of Martin et al. (1979). Sperm velocity, ATP
content and objective motility in ejaculates of subjective
motility >50% show values of 14.010.84 ym S-l;
4.8f 2 . 7 1~O - ~ Man d 54.0I7.4%, respectively. For
ejaculates with subjective motility ~ 5 0 %the~se values
are 8.412.4; 0.74f 0 . 3 6 1~ 0 - 7 a~n d 27.0+0.8%. No
significant changes in these characteristics were elicited
by freezing, though ATP content dropped to 50% after
thawing. These characteristics are highly associated
between them (pc0.05) and with some conventional
parameters of the routine seminogram such as sperm
motility, count, total spermatozoal number and
morphology. Additionally, sperm chromatin packing was
analyzed by decondensing sperm nuclei using a thiol
reducing agent. This parameter was not modified by
freezing and it may depend on prolonged epididymal
sperm storage during the non-breeding season.
Epididymal maturation then results in an excessive
disulphur bridging in sperm basic proteins, so that
hypermaturation with faultly male pronuclear formation
can follow.
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