Publication: Parafibromin expression in lung normal
tissue and carcinoma: its comparison with
clinicopathological parameters of carcinoma
Authors
Xia, Pu ; Wang, Wei ; Xu, Xiao-yan ; Wang, Jian-ping ; Takano, Yasuo ; Zheng, Hua-Chuan
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Publisher
Murcia: F. Hernández y Juan F. Madrid, Universidad de Murcia, Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Parafibromin is a protein encoded by the
hyperparathyroidism 2 oncosuppressor gene and its
down-regulated expression is involved in the
pathogenesis of parathyroid, gastric and colorectal
carcinomas. To clarify the roles of parafibromin
expression in lung carcinomas, it was examined by
immunohistochemistry and in situ hybridization on
tissue microarray containing lung carcinomas (n=144)
and normal lung tissue (n=20), with a comparison to
clinicopathological parameters of carcinomas. Lung
carcinoma cell lines and tissues were studied for
parafibromin expression by Western blot and RT-PCR.
Down-regulated expression of parafibromin mRNA was
found in lung carcinoma in comparison with matched
normal tissue (p<0.05). Parafibromin protein was found
in the cilia and nuclei of pseudo-stratified columnar
epithelium, and the nuclei of lung carcinoma. According
to immunostaining and in situ hybridization, there was
no difference in parafibromin expression between
histological subtypes of lung carcinoma (p>0.05). The
Kaplan-Meier method indicated that nuclear
parafibromin expression was positively correlated with
adenocarcinoma patients (p<0.05). Down-regulated
parafibromin mRNA expression might play an important
role in lung carcinogenesis, but not in its histogenesis.
Strong parafibromin expression in cilia of the pseudostratified
columnar epithelium indicated its possible
involvement in cell mobility. Parafibromin expression
could be employed to indicate the favorable prognosis of
patients with adenocarcinoma.
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