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Metabolomic changes in the rat retina after optic nerve crush

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dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.authorLahoz, Agustín
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorSobrado Calvo, Paloma
dc.contributor.authorPiquer Gil, Marina
dc.contributor.authorDíaz Llopis, Manuel
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.authorMullor, José L.
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.otherFacultades de la UMU::Facultad de Medicina
dc.date.accessioned2026-01-27T08:18:44Z
dc.date.available2026-01-27T08:18:44Z
dc.date.copyright© 2013 The Association for Research in Vision and Ophthalmology, Inc.
dc.date.issued2013-06-21
dc.description.abstractPurpose: To identify metabolic pathways and metabolites affected by optic nerve crush that can act as predictors of the disease or therapeutic targets. Methods: The left optic nerve of adult rats was intraorbitally crushed and retinas were dissected 24 hours or 14 days after the lesion (n = 10 per group). Metabolic profiling analysis was carried out by Metabolon, Inc. A total of 195 metabolites were unambiguously detected. Data were normalized and the regulated metabolites were identified after comparing the different conditions. Metabolite concentration changes were analyzed using single and multivariate statistical analysis to detect discriminatory metabolites. Functional clustering and meta-analysis of the regulated metabolites was run through the Metacore platform. Results: Comparison of 24 hours versus control, 14 days versus control samples, and 24 hours versus 14 days identified 9, 19, and 32 regulated metabolites, respectively. Single and multivariate analysis identified a total of 27 and 36 metabolites to discriminate between control and 14 days and between 24 hours and 14 days, respectively. Enrichment analysis showed alterations in the amino acid, carbohydrate, and lipid metabolism, which were further linked to translation, oxidative stress, energy (glucose and tricarboxylic acid cycle), and apoptosis through ceramide pathways. Conclusions: Our analysis differentiates a set of metabolites that clearly discriminate control and early-injury samples from late-injury samples. These metabolites could have potential use as diagnostic molecules.
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dc.format.extent11
dc.identifier.citationInvest Ophthalmol Vis Sci. 2013 Jun 21;54(6):4249-59
dc.identifier.doihttps://doi.org/10.1167/iovs.12-11451
dc.identifier.eissn1552-5783
dc.identifier.issn0146-0404
dc.identifier.urihttp://hdl.handle.net/10201/194049
dc.languageeng
dc.publisherAssociation for Research in Vision and Ophthalmology
dc.relationSupported by the Spanish Ministry of Economy and Competitiveness (BFU2009-10808), Instituto de Salud Carlos III Fondo Europeo de Desarrollo Regional ‘‘Una manera de hacer Europa’’ (PI10/00187, PI11/02942), Conselleria de Educacion Generalitat Valenciana (ACOMP2011/136), Fundación Séneca (04446/GERM/07), ´Spanish Ministry of Education and Science (SAF-22010-10385), and Red Temática de Investigacion Cooperativa en Oftalmología (RD07/0062/0001)
dc.relation.publisherversionhttps://iovs.arvojournals.org/article.aspx?articleid=2189653
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAmino acid metabolism
dc.subjectCarbohydrate
dc.subjectOxidative stress
dc.subjectGlutathione
dc.subjectTranslation
dc.subjectCeramide
dc.subjectAxotomy
dc.subjectMultivariate data analysis
dc.subjectCentral nervous system
dc.subjectTrauma
dc.subject.odsObjetivo 3: Salud
dc.titleMetabolomic changes in the rat retina after optic nerve crush
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
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