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Diagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin-embedded samples

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dc.contributor.authorSánchez, J.
dc.contributor.authorGallego, M. C.
dc.contributor.authorCaro, M. R.
dc.contributor.authorSalinas, J.
dc.contributor.authorMartínez Cáceres, Carlos Manuel
dc.contributor.authorNavarro Cámara, José Antonio
dc.contributor.authorOrtega Hernández, Nieves
dc.contributor.authorBuendía Marín, Antonio Julián
dc.contributor.departmentAnatomía y Anatomía Patológica Comparadas
dc.date.accessioned2025-01-20T09:47:44Z
dc.date.available2025-01-20T09:47:44Z
dc.date.issued2009-08-08
dc.description© British Veterinary Association 2009. This document is the Published version of a Published Work that appeared in final form in Veterinary Record. To access the final edited and published work see https://doi.org/10.1136/vr.165.6.175es
dc.description.abstractA histological study was carried out on 58 formalinfixed and paraffin-embedded samples of placenta from sheep and goats that had aborted, and the placental lesions were graded. Sequential histological sections of each cotyledon were then immunostained with specific antibodies and used for PCR detection of Chlamydophila abortus, Coxiella burnetii, Salmonella Abortusovis, Brucella melitensis, Listeria monocytogenes and Toxoplasma gondii. Most of the cotyledons showed different degrees of placentitis. The proportional agreement between the two techniques was 0·879 (κvalue 0·746). C abortus was the most prevalent pathogen. Mixed infections were common.es
dc.formatapplication/pdfes
dc.format.extent4es
dc.identifier.citationVeterinary Record, 2009, Vol. 165, Issue 6, pp. 175-178
dc.identifier.doihttps://doi.org/10.1136/vr.165.6.175
dc.identifier.issnPrint: 0042-4900
dc.identifier.issnElectronic: 2042-7670
dc.identifier.urihttp://hdl.handle.net/10201/148788
dc.languageenges
dc.publisherWileyes
dc.relationThe study was supported by Fundación SENECA, grant code 0055-0/PI/04. NO is the recipient of a postdoctoral grant from CajaMurcia.es
dc.relation.publisherversionhttps://bvajournals.onlinelibrary.wiley.com/doi/10.1136/vr.165.6.175es
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.titleDiagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin-embedded sampleses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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relation.isAuthorOfPublicationc084ce85-206c-418d-8cec-7f85a0f34dc0
relation.isAuthorOfPublication.latestForDiscoverya11bfbe4-fe50-4173-a96d-aa785375a6be
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