Publication: Freeze-fracture cytochemistry,a new fracture-labeling method for topological analysis of biomembrane molecules
Loading...
Date
2000
Authors
Takizawa, T. ; Robinson, J.M.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Freeze-fracture cytochemistry allows
visualization of cellular and molecular characteristics of
biomembranes in situ. In this review, we discuss freezefracture
cytochemistry with special reference to a new
cytochemical labeling of replicas, the detergentdigestion
fracture-labeling technique. In this procedure,
unfixed cells are rapidly-frozen, freeze-fractured, and
physically stabilized by evaporated platinum/carbon.
The frozen cells are then removed from the freezefracture
apparatus to thaw and are subsequently treated
with detergents. After detergent-digestion, replicas are
labeled with cytochemical markers. We demonstrate that
the technique is a versatile tool for direct analysis of the
macromolecular architecture of biomembranes and
allows identification of particular intracellular
membrane organelles. In addition, we demonstrate the
application of ultrasmall gold to freeze-fracture
immunocytochemistry. Freeze-fracture cytochemistry is
a valuable technique for investigating topology and
dynamics of membrane molecules.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.