Publication:
CDC28 protein kinase regulatory subunit 1B (CKS1B) expression and genetic status analysis in oral squamous cell carcinoma

Simple item page
dc.contributor.authorMartín-Ezquerra, Gemma
dc.contributor.authorSalgado, Rocio
dc.contributor.authorToll, Agustí
dc.contributor.authorBaró, Teresa
dc.contributor.authorMojal, Sergi
dc.contributor.authorYébenes, Mireia
dc.contributor.authorGarcia-Muret, María Pilar
dc.contributor.authorSolé, Francesc
dc.contributor.authorAlameda Quitllet, Francesc
dc.contributor.authorEspinet, Blanca
dc.contributor.authorPujol, Ramón M.
dc.date.accessioned2015-11-30T18:23:49Z
dc.date.available2015-11-30T18:23:49Z
dc.date.issued2011
dc.description.abstractCKS1B is a member of the highly conserved cyclin kinase subunit 1 (CKS1) protein family which interacts with cyclin-dependent kinases and plays a critical role in cell cycle progression. In oral squamous cell carcinoma (OSCC), as in other malignancies, CKS1B overexpression has been correlated with reduced survival. To our knowledge, no studies evaluating the genetic status of CKS1B gene in OSCC have been reported. Herein, genetic and protein status of CKS1B were analyzed by immunohistochemical (IHC) and fluorescence in situ hybridization (FISH) techniques in a series of primary OSCC (n=51) and lymph node OSCC metastases samples (n=14). The observed results were compared with those obtained in either inflammatory (oral lichen planus [OLP]) (n=13) and premalignant oral mucosal lesions (oral leukoplakia) (n=16). A significant CKS1B overexpression was observed in OSCC and lymph node metastases samples than in OLP and oral leukoplakia (mean 70% vs 35%, p<0.001). CKS1B overexpression correlated with p27 loss of expression (p=0.0013) and SKP2 overexpression (p<0.00). FISH study disclosed statistical differences in both gene amplifications and gains between samples corresponding to OSCC and metastases from those of OLP and leukoplakia (p<0.001). Amplifications were present in 53% of OSCC samples and 33% of lymph node metastases vs 14% of oral leukoplakia and 0% of OLP biopsy specimens (p=0.002). Polysomies of chromosome 1 were seen in 46% of OSCC, 33% of ganglionar metastases, 14% of oral leukoplakia and 10% of OLP (p=0.036). Correlation of CKS1B overexpression and gains (both polysomies and amplifications) determined by FISH was statistically significant (p<0.001). Our results indicate that a high CKS1B expression is a common finding in primary OSCC which correlates with p27 low expression and SKP2 overexpression. This phenomenon may be due either to numerical (chromosome 1 polysomy) or structural (amplifications) CKS1B genetic abnormalities. This phenotypical and cytogenetic profile is not observed in premalignant or inflammatory oral mucosal lesions.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/46921
dc.languageenges
dc.publisherMurcia: Universidad de Murcia, Facultad de Biologíaes
dc.relation.ispartofHistology and histopathology, Vol. 26, nº 1 (2011)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectCarcinogenesises
dc.subjectSquamous cell carcinomaes
dc.subject.other616.5 - Piel. Dermatología clínicaes
dc.titleCDC28 protein kinase regulatory subunit 1B (CKS1B) expression and genetic status analysis in oral squamous cell carcinomaes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
CDC28 protein kinase regulatory subunit 1B (CKS1B).pdf
Size:
1005.4 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.35 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Histology and histopathology Vol.26, nº1 (2011)