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1H Nuclear Magnetic Resonance of pig seminal plasma reveals intra-ejaculate variation in metabolites

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dc.contributor.authorMateo Otero, Yentel
dc.contributor.authorFernández-López, Pol
dc.contributor.authorGil-Caballero, Sergi
dc.contributor.authorFernandez-Fuertes, Beatriz
dc.contributor.authorBonet, Sergi
dc.contributor.authorBarranco Cascales, Isabel
dc.contributor.authorYeste, Marc
dc.contributor.departmentMedicina y Cirugía Animal
dc.contributor.otherFacultad de Veterinaria
dc.date.accessioned2025-10-17T10:23:27Z
dc.date.available2025-10-17T10:23:27Z
dc.date.copyright©2020 by the authors
dc.date.issued2020-06-15
dc.description.abstractIn pigs, ejaculate is expelled in fractions, mainly the sperm-rich fraction (SRF) and the post-SRF (PSRF), which differ in both sperm content and origin. In addition, intra-ejaculate variability between fractions in terms of sperm reproductive characteristics has been previously reported, the highest sperm quality being observed in the first 10 mL of the SRF (SRF-P1). As seminal plasma (SP) composition has been purported to influence sperm physiology, the aim of this study was to profile pig SP metabolite composition and to find putative differences between the ejaculate portions (SRF-P1, the rest of SRF [SRF-P2], PSRF) and entire ejaculate (EE). To this end, ejaculates (n = 8, one per boar) were collected in fractions and SP was analyzed using 1H Nuclear Magnetic Resonance spectroscopy. We identified 19 metabolites present in all ejaculate portions and the EE, and reported correlations between the metabolites. Additionally, and for the first time in mammals, we found intra-ejaculate variability in the SP metabolites, observing different relative abundances in choline, glycerophosphocholine and glycine. Regarding their influence in sperm physiology, we hypothesize that these metabolites may explain the specific reproductive characteristics of each ejaculate portion. Finally, the reported SP metabolites could serve as a first steppingstone in the study of quality, functionality, and fertility biomarkers.
dc.formatapplication/pdf
dc.format.extent16
dc.identifier.citationBiomolecules 2020, 10, 906
dc.identifier.doihttps://doi.org/10.3390/biom10060906
dc.identifier.eissn2218-273X
dc.identifier.urihttp://hdl.handle.net/10201/167332
dc.languageeng
dc.publisherMDPI
dc.relationThe present study has been funded by the Ministry of Science and Innovation, Spain (Grants: RYC-2014-15581, AGL2017-88329-R and FJCI-2017-31689) and the Regional Government of Catalonia (Grants: 2019-SGR-1229 and 2020-FI-B-00412).The authors of this manuscript would like to thank AIM Iberica (Topigs Norsvin Iberica, Spain) for supplying the boar ejaculates.
dc.relation.publisherversionhttps://www.mdpi.com/2218-273X/10/6/906
dc.rightsAttribution 4.0 Internacional
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectNuclear magnetic resonance
dc.subjectSeminal plasma
dc.subjectMetabolites
dc.subjectPig
dc.subjectIntra-ejaculate variability
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.title1H Nuclear Magnetic Resonance of pig seminal plasma reveals intra-ejaculate variation in metabolites
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
relation.isAuthorOfPublication.latestForDiscovery3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
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