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Monitoring of airborne biological particles in outdoor atmosphere. Part 2: metagenomics applied to urban environments

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dc.contributor.authorNúñez, Andrés
dc.contributor.authorAmo de Paz, Guillermo
dc.contributor.authorRastrojo, Alberto
dc.contributor.authorGarcía, Ana M.
dc.contributor.authorAlcamí, Antonio
dc.contributor.authorGutiérrez-Bustillo, A. Montserrat
dc.contributor.authorMoreno, Diego A.
dc.contributor.departmentGenética y Microbiología
dc.date.accessioned2024-06-25T11:42:01Z
dc.date.available2024-06-25T11:42:01Z
dc.date.issued2016-11-15
dc.description© 2016 Sociedad Española de Microbiología. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Submitted, Accepted, Published, version of a Published Work that appeared in final form in International Microbiology. To access the final edited and published work see https://doi.org/10.2436/20.1501.01.265
dc.description.abstractThe air we breathe contains microscopic biological particles such as viruses, bacteria, fungi and pollen, some of them with relevant clinic importance. These organisms and/or their propagules have been traditionally studied by different disciplines and diverse methodologies like culture and microscopy. These techniques require time, expertise and also have some important biases. As a consequence, our knowledge on the total diversity and the relationships between the different biological entities present in the air is far from being complete. Currently, metagenomics and next-generation sequencing (NGS) may resolve this shortage of information and have been recently applied to metropolitan areas. Although the procedures and methods are not totally standardized yet, the first studies from urban air samples confirm the previous results obtained by culture and microscopy regarding abundance and variation of these biological particles. However, DNA-sequence analyses call into question some preceding ideas and also provide new interesting insights into diversity and their spatial distribution inside the cities. Here, we review the procedures, results and perspectives of the recent works that apply NGS to study the main biological particles present in the air of urban environments.es
dc.formatapplication/pdfes
dc.format.extent12es
dc.identifier.citationInternational Microbiology, 2016, Vol. 19 (2), pp. 69-80
dc.identifier.doihttps://doi.org/10.2436/20.1501.01.265
dc.identifier.issnPrint: 1139-6709
dc.identifier.issnElectronic: 1618-1905
dc.identifier.urihttp://hdl.handle.net/10201/142651
dc.languageenges
dc.publisherInstitut d'Estudis Catalanses
dc.relationThis study was funded by the Community of Madrid, Spain, under the AIRBIOTA-CM Program (S2013/MAE-2874). Proyecto Regional.es
dc.relation.publisherversionhttps://revistes.iec.cat/index.php/IM/article/view/142234
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBioaerosoles
dc.subjectAerobiomees
dc.subjectMetagenomicses
dc.subjectMicrobiologyes
dc.subjectNGSes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biologíaes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiologíaes
dc.titleMonitoring of airborne biological particles in outdoor atmosphere. Part 2: metagenomics applied to urban environmentses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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