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The Influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA

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dc.contributor.authorEncinar Hidalgo, José Antonio
dc.contributor.authorMolina Gallego, María Luisa
dc.contributor.authorPoveda Larrosa, José Antonio
dc.contributor.authorBarrera Olivares, Francisco Nicolás
dc.contributor.authorRenart Pérez, María Lourdes
dc.contributor.authorFernández Carvajal, Asia María
dc.contributor.authorGonzález Ros, José Manuel
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2024-07-04T11:15:24Z
dc.date.available2024-07-04T11:15:24Z
dc.date.issued2005-09-26
dc.description© 2005 Federation of European Biochemical Societies. This document is the Published version of a Published Work that appeared in final form in FEBS Letters. To access the final edited and published work see https://doi.org/10.1016/j.febslet.2005.08.038
dc.description.abstractThe lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440–446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1–125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditions.es
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dc.format.extent6es
dc.identifier.citationFEBS Letters, 2005, Vol. 579, Issue 23,pp. 5199-5204
dc.identifier.doihttps://doi.org/10.1016/j.febslet.2005.08.038
dc.identifier.issnPrint: 0014-5793
dc.identifier.issnElectronic: 1873-3468
dc.identifier.urihttp://hdl.handle.net/10201/142859
dc.languageenges
dc.publisherElsevieres
dc.relationSupported by grants from the Spanish DGI BFI2002-03410 and from the Agencia Valenciana de Ciencia y Tecnología 03/056. F.N.B. and M.L.R. were partly supported by predoctoral fellowships from the Ministerio de Educación y Ciencia of Spain, while M.L.M. had a predoctoral fellowship from the Generalitat Valenciana.es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0014579305010380
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectInfrared spectroscopyes
dc.subjectAmide I′ and II bandses
dc.subjectThermal denaturationes
dc.titleThe Influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsAes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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