Publication: Immunohistochemical localization
of senescence marker protein-30 (SMP30)
in the submandibular gland and ultrastructural changes
of the granular duct cells in SMP30 knockout mice
Authors
Ishii, K. ; Tsubaki, T. ; Fujita, K. ; Ishigami, A. ; Maruyama, N. ; Akita, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Senescence Marker Protein-30 (SMP30) is a
calcium-regulating protein that decreases in an
androgen-independent manner as aging occurs. An
enzyme-labeled antibody technique has demonstrated
that SMP30 localized to the ducts (granular, intercalated,
and striated ducts) of mouse submandibular glands.
Immunoelectronmicroscopy demonstrated that the
granular duct cells were strongly positive for SMP30,
but that pillar cells in the granular duct were negative for
the protein. In SMP30-knockout (KO) mice, the granular
ducts were smaller in diameter. Swelling of
mitochondria in the granular duct cells was observed;
however, this phenomenon was not observed in the pillar
cells. After administration of a-isoproterenol, a betaadrenergic
stimulant, a large numbers of small secretory
granules were present in the granular duct cells and an
expansion of the rough endoplasmic reticulum in
SMP30-wild type (WT) mice; in contrast, little change
was observed in SMP30-KO mice. These results suggest
that SMP30 may be closely related to a signal
transduction pathway in the granular duct cells of
submandibular glands.
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