Publication: Skin homeostasis during inflammation, a role for nerve growth factor
Loading...
Date
2008
Authors
Sivilia, S. ; Paradisi, M. ; D’Intino, G. ; Fernandez, M. ; Pirondi, S. ; Lorenzini, L. ; Calzà, L.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The skin is a neuroendocrine immune organ
in which many different molecules operate in autocrineparacrine
manner to guarantee tissue homeostatsis in
physiological and pathophysiological conditions. In this
paper we examined NGF and p75 receptor expression in
the skin, during CFA induced inflammation, in a timecourse
study. We also examined cutaneus innervation
and proliferation, by means of immunohistochemistry
and quantitative image analysis, RT-PCR and Western
blot. Spontaneous and evoked pain-behavior was also
measured in experimental rats. The main results can be
summarized as follows: 1). a peripheral sensory
neuropathy develops in this condition, as indicated by
thermal hyperalgesia, thus leading to a sensory
denervation of the hind-paw skin as indicated by
disappearance of CGRP and PGP9.5-IR fibers; 2). NGF
and p75 expression (mRNA and protein) increases in the
skin (keratinocytes) in the acute phase of CFA
inflammation; 3). at this stage, a higher proliferative
activity is observed in the skin, as defined by the
expression of cell cycle-associated protein Ki67; 4). in
the long-lasting chronic phase there is a further upregulation
of NFG and p75 expression in the skin; 5). trkA mRNA expression inversely correlates with p75
and NGF mRNA expression. These results suggest that
CFA chronic inflammation evolves from inflammation
to a small fibers sensory neuropathy and NGF seems to
play a role in both events.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.