Continuous intracellular pH measurement: Escherichia coli culture medium pH dependence

Lozano Terol, Gema; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia

Publication:
Continuous intracellular pH measurement: Escherichia coli culture medium pH dependence

dc.contributor.author	Lozano Terol, Gema
dc.contributor.author	Cánovas Díaz, Manuel
dc.contributor.author	Diego Puente, Teresa de
dc.contributor.author	Gallego Jara, Julia
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2021-11-04T11:09:39Z
dc.date.available	2021-11-04T11:09:39Z
dc.date.issued	2021-05-04
dc.description.abstract	E. coli is the most used host microorganism to high-valuable compounds production in current industry. Cells pH homeostasis is a crucial factor to grow in different environment maintaining an intracellular pH control, however, to our knowledge, there is not a study about E. coli continuous intracellular pH growing in a culture. In this study the intracellular E. coli pH has been determined for cultures growing with glucose or glycerol as carbon sources. To determine intracellular E. coli pH, a novel, simple and accurate method based on pGFPR01 plasmid (kindly provided by Dr. Joan Slonczewski) was employed (1). This vector expressed ratiometric pHluorin. Thus, fluorescence ratio 410/470 showed a perfect lineal dependence with intracellular pH between 5.5 and 9. The results showed a surprising high intracellular pH for E. coli glucose cultures, which raised values of 8.5-9 at exponential growth phase. However, glycerol cultures showed a major regulation of intracellular pH, keeping the values at 6.5-7.5 in exponential and stationary stages. These differences show the dependence between the intracellular pH and the carbon source. In addition, the measurement method developed is a very useful tool for the control of bioprocesses.	es
dc.format	application/pdf	es
dc.format.extent	1	es
dc.identifier.uri	http://hdl.handle.net/10201/113699
dc.language	eng	es
dc.relation	Nombre del proyecto: Desvelando la dinámica del acetiloma para biología sintética en E. coli. Código: RTI2018-094393-B-C21. Agencia/entidad financiadora: Ministerio de Ciencia, Innovación y Universidades. Ámbito Nacional. Proyectos I+D+i - Modalidades «Retos Investigación» (con financiación FEDER). Convocatoria 2018. Nombre del proyecto: Biotecnología de sistemas para la mejora de bioprocesos de producción de proteínas recombinantes en E. coli: integración de la regulación transcripcional y post-traduccional del metabolismo de carbono y nitrógeno. Código: 19236/PI/14. Agencia/entidad financiadora: Fundación Séneca. Ámbito regional. Ayudas a la realización de proyectos para el desarrollo de investigación científica y técnica por grupos competitivos. Convocatoria 2018.	es
dc.relation.ispartof	13th European Symposium on Biochemical Engineering Sciences 2021	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Escherichia col	es
dc.subject	pH	es
dc.subject	intracellular	es
dc.subject	fluorescence	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica	es
dc.title	Continuous intracellular pH measurement: Escherichia coli culture medium pH dependence	es
dc.type	info:eu-repo/semantics/other	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	57bf3537-1124-49ab-8774-bd0c3bdf1c28
relation.isAuthorOfPublication	b8be181a-7224-475a-8427-543751ef593c
relation.isAuthorOfPublication	0212fc0f-e15c-4b27-9a2d-116768a47044
relation.isAuthorOfPublication.latestForDiscovery	57bf3537-1124-49ab-8774-bd0c3bdf1c28