Publication: Proliferation and migration kinetics of stem cells in the rat fundic gland
Loading...
Date
1997
Authors
Yang, D.H. ; Tsuyama, S. ; Ge, Y. B. ; Wakamatsu, D. ; Ohmori, J. ; Murata, F.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The proliferation and migration of stem cells
in the developing and adult rat fundic gland have been
studied using BrdU immunohistochemistry and BrdUGSA
11 (Griffonia-simplicifolia agglutinin-11) double
staining.
In the developing rat fundic gland, stem cells were
first scattered throughout al1 levels of the epithelia and
then concentrated in the depth of the pits. With the
elongation and maturation of the fundic glands, stem
ceils left the gland base and moved upward. By 4 weeks
after birth, the development of the fundic gland was
completed and stem cells were confined to a narrow
proliferative zone in the isthmus, reaching the adult
distribution pattern.
In the adult rat fundic gland, stem cells in the isthmus
differentiated and migrated upward and downward,
replacing the surface mucous cells and glandular cells
respectively. For upward migration, it took about one
week for stem cells to migrate from the isthmus to the
surface. For downward migration, it took about two
weeks for stem cells to migrate from the isthmus to the
neck, and it took 30-36 weeks to reach the gland unit's
blind end. FinaJly stem cells were lost at the deepest
leve1 of the glands.
The results obtained by simple topographical
distribution in the present experiment agreed well with
those obtained by quantitative analysis, suggesting the
usefulness of BrdU immunohistochemistry for cell
kinetic studies.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.